Characterisation of Erysipelothrix rhusiopathiae isolates from pigs associated with vaccine breakdowns

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Show simple item record Eamens, GJ Forbes, WA Djordjevic, SP 2010-05-28T09:45:56Z 2006-01
dc.identifier.citation Veterinary Microbiology, 2006, 115 (4), pp. 329 - 338
dc.identifier.issn 0378-1135
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Swine erysipelas vaccines are routinely used to protect pigs against peracute and acute/urticarial forms of Erysipelothrix. Between 1995 and 1998, 34 swine herds across four Australian states experienced vaccine failure. Forty-four isolates of Erysipelothrix rhusiopathiae of serovars 2, 1a, 1b and 1b × 21 were recovered from 15 of these 34 vaccine breakdown herds. These isolates were characterised by restriction fragment length polymorphism (RFLP) analyses using RsaI and AluI on whole cell DNA and for the presence of plasmid DNA. Results were compared with those of 20 isolates from 16 herds unaffected by vaccine breakdown and 13 isolates representing 10 reference strains. The majority of breakdown herds possessed isolates of serovar 2 (9/15 herds), followed by serovar 1a (5 herds). No geographic predominance of a single serovar was evident. The identification of 10 RsaI profiles from whole cell DNA among the 44 isolates from 15 breakdown herds indicated that a single, new clonal lineage of E. rhusiopathiae was not responsible for vaccine failure. RsaI RFLP analyses detected a further 14 distinct profiles among 20 field strains unassociated with vaccine breakdowns, and none matched profiles of the 10 serovar reference strains for serovars 1a, 1b, 2 or 21. This technique is recommended for epidemiological studies of E. rhusiopathiae strains.
dc.publisher Elsevier BV
dc.relation.isbasedon 10.1016/j.vetmic.2006.02.015
dc.title Characterisation of Erysipelothrix rhusiopathiae isolates from pigs associated with vaccine breakdowns
dc.type Journal Article
dc.parent Veterinary Microbiology
dc.journal.volume 4
dc.journal.volume 115
dc.journal.number en_US
dc.journal.number 4 en_US
dc.publocation Netherlands en_US
dc.identifier.startpage 329 en_US
dc.identifier.endpage 338 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 070707 Veterinary Microbiology (Excl. Virology)
dc.personcode 107126
dc.percentage 100 en_US Veterinary Microbiology (excl. Virology) en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords Erysipelothrix rhusiopathiae
dc.description.keywords Restriction fragment length polymorphism
dc.description.keywords Plasmids
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)

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