The effect of the microtubule inhibitor tubulozole-C on the tegument of triclabendazole-susceptible and triclabendazole-resistant Fasciola hepatica.

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dc.contributor.author Robinson, MW
dc.contributor.author Trudgett, A
dc.contributor.author Hoey, EM
dc.contributor.author Fairweather, I
dc.date.accessioned 2010-05-28T09:46:11Z
dc.date.issued 2003-09
dc.identifier.citation Parasitology research, 2003, 91 (2), pp. 117 - 129
dc.identifier.issn 0932-0113
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8902
dc.description.abstract The benzimidazole derivative, triclabendazole is the main drug used against Fasciola hepatica, although its precise mode of action remains to be fully determined. Previous studies have suggested that triclabendazole acts as a microtubule inhibitor in the same manner as tubulozole-C. Consequently, flukes from triclabendazole-susceptible and triclabendazole-resistant isolates were treated with tubulozole-C (1x10(-6 )M) in vitro and changes in tegumental morphology and tubulin distribution within the tegument were monitored by scanning and transmission electron microscopy, together with tubulin immunocytochemistry. Tubulozole-C caused severe disruption within the tegument of triclabendazole-susceptible flukes. Tubulin immunoreactivity diminished within the tegumental syncytium of the triclabendazole-susceptible flukes following treatment with tubulozole-C. In contrast, tubulozole-C caused only minor disruption of the tegument in triclabendazole-resistant F. hepatica and did not significantly alter the pattern of tubulin immunostaining within the tegumental syncytium. The results of the present study indicate that tubulozole-C and triclabendazole share the same target molecule and that triclabendazole-resistant flukes are also cross-resistant to tubulozole-C.
dc.format Print-Electronic
dc.language eng
dc.relation.isbasedon 10.1007/s00436-003-0953-z
dc.title The effect of the microtubule inhibitor tubulozole-C on the tegument of triclabendazole-susceptible and triclabendazole-resistant Fasciola hepatica.
dc.type Journal Article
dc.parent Parasitology research
dc.journal.volume 2
dc.journal.volume 91
dc.journal.number 2 en_US
dc.publocation Berlin, Germany en_US
dc.identifier.startpage 117 en_US
dc.identifier.endpage 129 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0707 Veterinary Sciences
dc.for 0605 Microbiology
dc.personcode 100777
dc.percentage 50 en_US
dc.classification.name Veterinary Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.location.activity Germany
dc.description.keywords NA en_US
dc.description.keywords NA
dc.description.keywords Animals
dc.description.keywords Antiplatyhelmintic Agents
dc.description.keywords Benzimidazoles
dc.description.keywords Dioxolanes
dc.description.keywords Drug Resistance
dc.description.keywords Fasciola hepatica
dc.description.keywords Fascioliasis
dc.description.keywords Microscopy, Electron
dc.description.keywords Microscopy, Electron, Scanning
dc.description.keywords Microtubules
dc.description.keywords Parasitic Sensitivity Tests
dc.description.keywords Rats
dc.description.keywords Tubulin
dc.description.keywords Animals
dc.description.keywords Rats
dc.description.keywords Microtubules
dc.description.keywords Fascioliasis
dc.description.keywords Dioxolanes
dc.description.keywords Benzimidazoles
dc.description.keywords Tubulin
dc.description.keywords Microscopy, Electron
dc.description.keywords Parasitic Sensitivity Tests
dc.description.keywords Antiplatyhelmintic Agents
dc.description.keywords Microscopy, Electron, Scanning
dc.description.keywords Drug Resistance
dc.description.keywords Fasciola hepatica
dc.description.keywords Animals
dc.description.keywords Rats
dc.description.keywords Microtubules
dc.description.keywords Fascioliasis
dc.description.keywords Dioxolanes
dc.description.keywords Benzimidazoles
dc.description.keywords Tubulin
dc.description.keywords Microscopy, Electron
dc.description.keywords Parasitic Sensitivity Tests
dc.description.keywords Antiplatyhelmintic Agents
dc.description.keywords Microscopy, Electron, Scanning
dc.description.keywords Drug Resistance
dc.description.keywords Fasciola hepatica
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science


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