Genetic diversity amongst isolates of Neospora caninum, and the development of a multiplex assay for the detection of distinct strains

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Show simple item record Al-Qassab, S Reichel, MP Ivens, A Ellis, JT 2010-05-28T09:46:13Z 2009-06
dc.identifier.citation Molecular and Cellular Probes, 2009, 23 (3-4), pp. 132 - 139
dc.identifier.issn 0890-8508
dc.identifier.other C1 en_US
dc.description.abstract Infection with Neospora caninum is regarded as a significant cause of abortion in cattle. Despite the economic impact of this infection, relatively little is known about the biology of this parasite. In this study, mini and microsatellite DNAs were detected in the genome of N. caninum and eight loci were identified that each contained repetitive DNA which was polymorphic among different isolates of this parasite. A multiplex PCR assay was developed for the detection of genetic variation within N. caninum based on length polymorphism associated with three different repetitive markers. The utility of the multiplex PCR was demonstrated in that it was able to distinguish amongst strains of N. caninum used as either vaccine or challenge strains in animal vaccination experiments and that it could genotype N. caninum associated with naturally acquired infections of animals. The multiplex PCR is simple, rapid, informative and sensitive and should provide a valuable tool for further studies on the epidemiology of N. caninum in different host species. © 2009 Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.hasversion Accepted manuscript version en_US
dc.relation.isbasedon 10.1016/j.mcp.2009.01.006
dc.title Genetic diversity amongst isolates of Neospora caninum, and the development of a multiplex assay for the detection of distinct strains
dc.type Journal Article
dc.description.version Published
dc.parent Molecular and Cellular Probes
dc.journal.volume 3-4
dc.journal.volume 23
dc.journal.number 3-4 en_US
dc.publocation London, UK en_US
dc.identifier.startpage 132 en_US
dc.identifier.endpage 139 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 998123
dc.personcode 105509
dc.personcode 910945
dc.percentage 100 en_US Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity ISI:000266913300002 en_US
dc.description.keywords Cattle
dc.description.keywords Genotyping
dc.description.keywords Multiplex
dc.description.keywords Neospora caninum
dc.description.keywords PCR
dc.description.keywords Repetitive DNA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Open Access 2015-04-15 12:23:47.074767+10
pubs.consider-herdc true
utslib.collection.history General (ID: 2)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)

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