Bacterial origin recognition complexes direct assembly of higher-order DnaA oligomeric structures

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Show simple item record Miller, DT Grimwade, JE Betteridge, T Rozgaja, T Torgue, JJ Leonard, AC 2010-05-28T09:49:36Z 2009-01
dc.identifier.citation Proceedings Of The National Academy Of Sciences ..., 2009, 106 (44), pp. 18479 - 18484
dc.identifier.issn 0027-8424
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Eukaryotic initiator proteins form origin recognition complexes (ORCs) that bind to replication origins during most of the cell cycle and direct assembly of prereplication complexes (pre-RCs) before the onset of S phase. In the eubacterium Escherichia coli, there is a temporally similar nucleoprotein complex comprising the initiator protein DnaA bound to three high-affinity recognition sites in the unique origin of replication, oriC. At the time of initiation, this high-affinity DnaAoriC complex (the bacterial ORC) accumulates additional DnaA that interacts with lower-affinity sites in oriC, forming a pre-RC. In this paper, we investigate the functional role of the bacterial ORC and examine whether it mediates low-affinity DnaAoriC interactions during pre-RC assembly. We report that E. coli ORC is essential for DnaA occupation of low-affinity sites. The assistance given by ORC is directed primarily to proximal weak sites and requires oligomerization-proficient DnaA. We propose that in bacteria, DnaA oligomers of limited length and stability emerge from single high-affinity sites and extend toward weak sites to facilitate their loading as a key stage of prokaryotic pre-RC assembly.
dc.publisher National Academy of Sciences
dc.relation.isbasedon 10.1073/pnas.0909472106
dc.title Bacterial origin recognition complexes direct assembly of higher-order DnaA oligomeric structures
dc.type Journal Article
dc.parent Proceedings Of The National Academy Of Sciences ...
dc.journal.volume 44
dc.journal.volume 106
dc.journal.number 44 en_US
dc.publocation USA en_US
dc.identifier.startpage 18479 en_US
dc.identifier.endpage 18484 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 106539
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords DNA replication, E. coli, ORC, oriC, pre-RC en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)
utslib.collection.history Uncategorised (ID: 363)

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