Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa

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dc.contributor.author Semmler, ABT
dc.contributor.author Whitchurch, CB
dc.contributor.author Leech, AJ
dc.contributor.author Mattick, JS
dc.date.accessioned 2010-05-28T09:50:07Z
dc.date.issued 2000-06
dc.identifier.citation Microbiology, 2000, 146 (6), pp. 1321 - 1332
dc.identifier.issn 1350-0872
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/9507
dc.description.abstract Transposon mutagenesis was used to identify a new locus required for twitching motility in Pseudomonas aeruginosa. Four Tn5-B21 mutants which lacked twitching motility and a fifth which exhibited impaired motility were found to map to the same KpnI restriction fragment at approximately 40 min on the P. aeruginosa genome. Cloning and sequencing studies showed that all five transposon insertions occurred within the same 2.8 kb ORF, which was termed fimV. The product of this gene has a putative peptidoglycan-binding domain, predicted transmembrane domains, a highly acidic C terminus and anomalous electrophoretic migration, indicating unusual primary or secondary structure. The P. aeruginosa genome also possesses a paralogue of fimV. Homologues of fimV were also found in the sequenced genomes of the other type-IV-fimbriated bacteria Neisseria gonorrhoeae, Neisseria meningitidis, Legionella pneumophila and Vibrio cholerae, but not in those of other bacteria which lack type IV fimbriae. A fimV homologue was also found in the genome sequence of Shewanella putrefaciens, along with many other homologues of type IV fimbrial genes, indicating that this bacterium is also likely to produce type IV fimbriae. Wild-type twitching motility was restored to fimV mutants by complementation in a dosage-dependent manner. Overexpression of fimV resulted in an unusual phenotype where the cells were massively elongated and migrated in large convoys at the periphery of the colony. It is suggested that FimV may be involved in remodelling of the peptidoglycan layer to enable assembly of the type IV fimbrial structure and machinery.
dc.language eng
dc.title Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa
dc.type Journal Article
dc.parent Microbiology
dc.journal.volume 6
dc.journal.volume 146
dc.journal.number en_US
dc.publocation Reading, UK en_US
dc.identifier.startpage 1321 en_US
dc.identifier.endpage 1332 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 1108 Medical Microbiology
dc.personcode 103745
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000087598700011 en_US
dc.location.activity ISI:000087598700011
dc.description.keywords Type-4 Fimbrial Biogenesis; Gram-negative Bacteria; Iv Pilus Biogenesis; Protein Secretion; Bacteroides-nodosus; Myxococcus-xanthus; Cloning Vectors; Polar Pili; Expression; Sequence en_US
dc.description.keywords Type-4 Fimbrial Biogenesis
dc.description.keywords Gram-negative Bacteria
dc.description.keywords Iv Pilus Biogenesis
dc.description.keywords Protein Secretion
dc.description.keywords Bacteroides-nodosus
dc.description.keywords Myxococcus-xanthus
dc.description.keywords Cloning Vectors
dc.description.keywords Polar Pili
dc.description.keywords Expression
dc.description.keywords Sequence
dc.description.keywords Animals
dc.description.keywords Humans
dc.description.keywords Pseudomonas aeruginosa
dc.description.keywords Bacteria
dc.description.keywords Restriction Mapping
dc.description.keywords Cloning, Molecular
dc.description.keywords Genetic Complementation Test
dc.description.keywords Mutagenesis, Insertional
dc.description.keywords Virulence
dc.description.keywords Phenotype
dc.description.keywords Plasmids
dc.description.keywords Molecular Sequence Data
dc.description.keywords Movement
dc.description.keywords Genes, Bacterial
dc.description.keywords Fimbriae
dc.description.keywords Fimbriae
dc.description.keywords Pseudomonas aeruginosa
dc.description.keywords Pseudomonas aeruginosa
dc.description.keywords Surface translocation
dc.description.keywords Surface translocation
dc.description.keywords Twitching motility
dc.description.keywords Twitching motility
dc.description.keywords Type IV pili
dc.description.keywords Type IV pili
dc.description.keywords Fimbriae
dc.description.keywords Pseudomonas aeruginosa
dc.description.keywords Surface translocation
dc.description.keywords Twitching motility
dc.description.keywords Type IV pili
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10


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