Lysozyme sorption in hydrogel contact lenses

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Show simple item record Garrett, Q Garrett, RW Milthorpe, BK 2010-05-28T09:50:12Z 1999
dc.identifier.citation Investigative Ophthalmology and Visual Science, 1999, 40 (5), pp. 897 - 903
dc.identifier.issn 0146-0404
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract PURPOSE. To examine the processes involved in formation of protein deposits on hydrogel contact lenses. METHODS. The adsorption and/or penetration of lysozyme on or into three types of contact lenses, etafilcon A, vifilcon A, and tefilcon, were investigated in vitro using a radiolabel- tracer technique, x-ray photoelectron spectroscopy, and laser scanning confocal microscopy. RESULTS. Binding of lysozyme to high-water-content, ionic contact lenses (etafilcon A and vifilcon A) was dominated by a penetration process. The extent of this penetration was a function of charge density of the lenses, so that there was a higher degree of penetration of lysozyme in etafilcon A than in vifilcon A lenses. In contrast, the binding of lysozyme to tefilcon lenses was a surface adsorption process. The adsorption and desorption kinetics showed similar trends to those found in human serum albumin (HSA) adsorption on lens surfaces. However, the extent of lysozyme adsorption on tefilcon is much higher than HSA adsorption, probably because of the self-association of lysozyme on the tefilcon lens surface. Furthermore, either penetration or adsorption of lysozyme involved reversible and irreversible processes and were both time dependent. CONCLUSIONS. Binding of lysozyme to hydrogel lenses involves surface adsorption or matrix penetration. These processes may be reversible or irreversible. The properties of the lens materials, such as charge density (ionicity) and porosity (water content) of the lenses, determine the type and rates of these processes.
dc.language eng
dc.title Lysozyme sorption in hydrogel contact lenses
dc.type Journal Article
dc.parent Investigative Ophthalmology and Visual Science
dc.journal.volume 5
dc.journal.volume 40
dc.journal.number 5 en_US
dc.publocation USA en_US
dc.identifier.startpage 897 en_US
dc.identifier.endpage 903 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 1108 Medical Microbiology
dc.personcode 105631
dc.percentage 100 en_US Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)

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