linG, a new integron-associated gene cassette encoding aLincosamide Nucleotidyltransferase

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Show simple item record Levings, RS Hall, RM Lightfoot, D Djordjevic, SP 2010-05-28T09:50:25Z 2006-01
dc.identifier.citation Antimicrobial agents and chemotherapy, 2006, 50 (10), pp. 3514 - 3515
dc.identifier.issn 0066-4804
dc.identifier.other C4 en_US
dc.description.abstract We have identified a second lin gene in a gene cassette. This cassette was recovered from a multiply antibiotic-resistant Salmonella enterica serovar Stanley strain (SRC54) isolated in 2001 from a traveler who had recently returned from Thailand. This strain was resistant to chloramphenicol, gentamicin, kanamycin, spectinomycin, streptomycin, sulfathiazole, and tetracycline but susceptible to ampicillin and nalidixic acid at levels described previously (6). It displayed intermediate resistance to ciprofloxacin. The gene cassette array was amplified by using standard primers (L2 and R1) in the 5' conserved sequence and 3' conserved sequence of class 1 integrons, and the 2.25-kb amplicon was cloned into pPCRscript and sequenced as previously described (see reference 6 for primer details). E. coli strain DH5{alpha} containing pPCR-Script with the cassette array was at least 10-fold more resistant to lincomycin (MIC, ?2,000 µg/ml) than DH5{alpha} containing only pPCR-Script (MIC, 180 µg/ml).
dc.format Esha Dutt
dc.publisher American Society for Microbiology
dc.relation.isbasedon 10.1128/AAC.00817-06
dc.title linG, a new integron-associated gene cassette encoding aLincosamide Nucleotidyltransferase
dc.type Journal Article
dc.parent Antimicrobial agents and chemotherapy
dc.journal.volume 10
dc.journal.volume 50
dc.journal.number 10 en_US
dc.publocation United States en_US
dc.identifier.startpage 3514 en_US
dc.identifier.endpage 3515 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 107126
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)

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