Roles of tyrosine-rich precursor glycoproteins and dityrosine-/DOPA-mediated protein crosslinking in oocyst wall assembly in the coccidian parasite, Eimeria maxima

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dc.contributor.author Belli, S
dc.contributor.author Wallach, M
dc.contributor.author Luxford, C
dc.contributor.author Davies, MJ
dc.contributor.author Smith, NC
dc.date.accessioned 2010-05-28T09:50:27Z
dc.date.issued 2003-01
dc.identifier.citation Eukaryotic Cell, 2003, 2 (3), pp. 456 - 464
dc.identifier.issn 1535-9778
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/9561
dc.description.abstract The oocyst wall of apicomplexan parasites protects them from the harsh external environment, preserving their survival prior to transmission to the next host. If oocyst wall formation could be disrupted, then logically, the cycle of disease transmission could be stopped, and strategies to control infection by several organisms of medical and veterinary importance such as Eimeria, Plasmodium, Toxoplasma, Cyclospora, and Neospora could be developed. Here, we show that two tyrosine-rich precursor glycoproteins, gam56 and gam82, found in specialized organelles (wall-forming bodies) in the sexual stage (macrogamete) of Eimeria maxima are proteolytically processed into smaller glycoproteins, which are then incorporated into the developing oocyst wall. The identification of high concentrations of dityrosine and 3,4-dihydroxyphenylalanine (DOPA) in oocyst extracts by high-pressure liquid chromatography, together with the detection of a UV autofluorescence in intact oocysts, implicates dityrosine- and possibly DOPA-protein cross-links in oocyst wall hardening. In addition, the identification of peroxidase activity in the wall-forming bodies of macrogametes supports the hypothesis that dityrosine- and DOPA-mediated cross-linking might be an enzyme-catalyzed event. As such, the mechanism of oocyst wall formation in Eimeria, is analogous to the underlying mechanisms involved in the stabilization of extracellular matrices in a number of organisms, widely distributed in nature, including insect
dc.publisher American Society for Microbiology
dc.relation.isbasedon 10.1128/EC.2.3.456¿464.2003
dc.title Roles of tyrosine-rich precursor glycoproteins and dityrosine-/DOPA-mediated protein crosslinking in oocyst wall assembly in the coccidian parasite, Eimeria maxima
dc.type Journal Article
dc.parent Eukaryotic Cell
dc.journal.volume 3
dc.journal.volume 2
dc.journal.number 3 en_US
dc.publocation United States en_US
dc.identifier.startpage 456 en_US
dc.identifier.endpage 464 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 110309 Infectious Diseases
dc.personcode 960087
dc.personcode 021128
dc.percentage 100 en_US
dc.classification.name Infectious Diseases en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)


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