Three-Dimensional Neural Differentiation of Mesenchymal Stem Cells

Gomila Pelegri, Neus

Three-Dimensional Neural Differentiation of Mesenchymal Stem Cells

Gomila Pelegri, Neus

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Publication Type:: Thesis
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Gomila Pelegri, Neus
dc.date.accessioned	2024-04-16T02:50:31Z
dc.date.available	2024-04-16T02:50:31Z
dc.date.issued	2023
dc.identifier.uri	http://hdl.handle.net/10453/177959
dc.description	University of Technology Sydney. Faculty of Science.	en_US.UTF-8
dc.description.abstract	Neurological disorders are the leading cause of disability and death worldwide, with over 9 million deaths annually and a large socioeconomic impact. To date, most neurological conditions remain poorly understood, incurable and difficult to treat, and research translation has been limited by a lack of physiologically relevant models. Tissue engineering provides an avenue to create more physiologically relevant models. Healthy neural cells are difficult to source, and Human Adipose-derived stem cells (hADSCs) are an interesting candidate to study and treat neurological conditions, given that they are relatively easy to obtain, can be autologous and can differentiate into neural cells. This thesis describes the investigation of hydrogels as suitable 3D models to differentiate hADSCs into neural cells and test different commercially available supplements (B27, C1, and N2) to enhance differentiation. Overall, the results showed that B27, C1 and N2 were suitable supplements to initiate neural differentiation of hADSCs both in 2D and 3D. Results showed that PEG-based hydrogels at 1.1kPa with RGB and YIGSR peptides successfully initiated and enhanced neural differentiation. These findings suggest that PEG-based hydrogels in combination with B27, C1 and N2 supplements are a good combination to create hADSCs neural differentiation models.	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en_US	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/177959/1/thesis.pdf
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.rights	© 2023 Neus Gomila Pelegri
dc.rights	au.edu.uts.lib/cph
dc.title	Three-Dimensional Neural Differentiation of Mesenchymal Stem Cells	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	open_access	*

Abstract:

Neurological disorders are the leading cause of disability and death worldwide, with over 9 million deaths annually and a large socioeconomic impact. To date, most neurological conditions remain poorly understood, incurable and difficult to treat, and research translation has been limited by a lack of physiologically relevant models. Tissue engineering provides an avenue to create more physiologically relevant models. Healthy neural cells are difficult to source, and Human Adipose-derived stem cells (hADSCs) are an interesting candidate to study and treat neurological conditions, given that they are relatively easy to obtain, can be autologous and can differentiate into neural cells. This thesis describes the investigation of hydrogels as suitable 3D models to differentiate hADSCs into neural cells and test different commercially available supplements (B27, C1, and N2) to enhance differentiation. Overall, the results showed that B27, C1 and N2 were suitable supplements to initiate neural differentiation of hADSCs both in 2D and 3D. Results showed that PEG-based hydrogels at 1.1kPa with RGB and YIGSR peptides successfully initiated and enhanced neural differentiation. These findings suggest that PEG-based hydrogels in combination with B27, C1 and N2 supplements are a good combination to create hADSCs neural differentiation models.

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http://hdl.handle.net/10453/177959