The expression difference of AChE, BChE, PON-1 and FOS mRNA in rats died of acute phorate poisoning

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Journal Article
Chinese Journal of Forensic Medicine, 2016, 31 (3), pp. 249 - 252
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Objective To investigate mRNA expression of the related genes (AChE,BChE,PON-1 and FOS) in SD rats died of acute phorate poisoning, and screen the significant organs and genes in the detection of death. Methods 12 grown-up female SD rats were divided into the acute poisoning group and the control group. Organ samples( heart, liver, lung, brain, small intestine) were collected respectively, and total RNA were isolated to be reversely transcribed to cDNA with gene primers of AChE, BChE, PON-1, FOS and interior label β-actin. The Real Time PCR was used to detect the comparative expression differences of them and the data of all the groups were analyzed statistically. Results In liver, the expression of AChE, BChE and FOS mRNA increased (P<0.05), while the expression of AChE mRNA decreased in lung and brain (P<0.05). Conclusion Liver and AChE could be served as the target organ and the gene expression mark for death by acute phorate poisoning respectively, which could also be set as the reference of the diagnose and death detection from acute phorate poisoning.
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