Genetic evolution of the neuraminidase of influenza a (H3N2) viruses from 1968 to 2009 and its correspondence to haemagglutinin evolution

Westgeest, KB; de Graaf, M; Fourment, M; Bestebroer, TM; van Beek, R; Spronken, MIJ; de Jong, JC; Rimmelzwaan, GF; Russell, CA; Osterhaus, ADME; Smith, GJD; Smith, DJ; Fouchier, RAM

Genetic evolution of the neuraminidase of influenza a (H3N2) viruses from 1968 to 2009 and its correspondence to haemagglutinin evolution

Westgeest, KB de Graaf, M Fourment, M

Bestebroer, TM van Beek, R Spronken, MIJ de Jong, JC Rimmelzwaan, GF Russell, CA Osterhaus, ADME Smith, GJD Smith, DJ Fouchier, RAM

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Publication Type:: Journal Article
Citation:: Journal of General Virology, 2012, 93 (PART 9), pp. 1996 - 2007
Issue Date:: 2012-09-01

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Field	Value	Language
dc.contributor.author	Westgeest, KB	en_US
dc.contributor.author	de Graaf, M	en_US
dc.contributor.author	Fourment, M https://orcid.org/0000-0001-8153-9822	en_US
dc.contributor.author	Bestebroer, TM	en_US
dc.contributor.author	van Beek, R	en_US
dc.contributor.author	Spronken, MIJ	en_US
dc.contributor.author	de Jong, JC	en_US
dc.contributor.author	Rimmelzwaan, GF	en_US
dc.contributor.author	Russell, CA	en_US
dc.contributor.author	Osterhaus, ADME	en_US
dc.contributor.author	Smith, GJD	en_US
dc.contributor.author	Smith, DJ	en_US
dc.contributor.author	Fouchier, RAM	en_US
dc.date.issued	2012-09-01	en_US
dc.identifier.citation	Journal of General Virology, 2012, 93 (PART 9), pp. 1996 - 2007	en_US
dc.identifier.issn	0022-1317	en_US
dc.identifier.uri	http://hdl.handle.net/10453/114750
dc.description.abstract	Each year, influenza viruses cause epidemics by evading pre-existing humoral immunity through mutations in the major glycoproteins: the haemagglutinin (HA) and the neuraminidase (NA). In 2004, the antigenic evolution of HA of human influenza A (H3N2) viruses was mapped (Smith et al., Science 305, 371-376, 2004) from its introduction in humans in 1968 until 2003. The current study focused on the genetic evolution of NA and compared it with HA using the dataset of Smith and colleagues, updated to the epidemic of the 2009/2010 season. Phylogenetic trees and genetic maps were constructed to visualize the genetic evolution of NA and HA. The results revealed multiple reassortment events over the years. Overall rates of evolutionary change were lower for NA than for HA1 at the nucleotide level. Selection pressures were estimated, revealing an abundance of negatively selected sites and sparse positively selected sites. The differences found between the evolution of NA and HA1 warrant further analysis of the evolution of NA at the phenotypic level, as has been done previously for HA. © 2012 SGM.	en_US
dc.relation.ispartof	Journal of General Virology	en_US
dc.relation.isbasedon	10.1099/vir.0.043059-0	en_US
dc.subject.classification	Virology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Neuraminidase	en_US
dc.subject.mesh	Viral Proteins	en_US
dc.subject.mesh	Hemagglutinin Glycoproteins, Influenza Virus	en_US
dc.subject.mesh	Evolution, Molecular	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Recombination, Genetic	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Influenza, Human	en_US
dc.subject.mesh	Influenza A Virus, H3N2 Subtype	en_US
dc.subject.mesh	Selection, Genetic	en_US
dc.subject.mesh	Mutation Rate	en_US
dc.title	Genetic evolution of the neuraminidase of influenza a (H3N2) viruses from 1968 to 2009 and its correspondence to haemagglutinin evolution	en_US
dc.type	Journal Article
utslib.citation.volume	PART 9	en_US
utslib.citation.volume	93	en_US
utslib.for	060506 Virology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	PART 9	en_US
pubs.publication-status	Published	en_US
pubs.volume	93	en_US

Abstract:

Each year, influenza viruses cause epidemics by evading pre-existing humoral immunity through mutations in the major glycoproteins: the haemagglutinin (HA) and the neuraminidase (NA). In 2004, the antigenic evolution of HA of human influenza A (H3N2) viruses was mapped (Smith et al., Science 305, 371-376, 2004) from its introduction in humans in 1968 until 2003. The current study focused on the genetic evolution of NA and compared it with HA using the dataset of Smith and colleagues, updated to the epidemic of the 2009/2010 season. Phylogenetic trees and genetic maps were constructed to visualize the genetic evolution of NA and HA. The results revealed multiple reassortment events over the years. Overall rates of evolutionary change were lower for NA than for HA1 at the nucleotide level. Selection pressures were estimated, revealing an abundance of negatively selected sites and sparse positively selected sites. The differences found between the evolution of NA and HA1 warrant further analysis of the evolution of NA at the phenotypic level, as has been done previously for HA. © 2012 SGM.

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http://hdl.handle.net/10453/114750