In vitro cell integrated impactor deposition methodology for the study of aerodynamically relevant size fractions from commercial pressurised metered dose inhalers

Haghi, M; Traini, D; Young, P

In vitro cell integrated impactor deposition methodology for the study of aerodynamically relevant size fractions from commercial pressurised metered dose inhalers

Haghi, M

Traini, D

Young, P

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Publication Type:: Journal Article
Citation:: Pharmaceutical Research, 2014, 31 (7), pp. 1779 - 1787
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Haghi, M https://orcid.org/0000-0002-3362-1845	en_US
dc.contributor.author	Traini, D https://orcid.org/0000-0002-7173-017X	en_US
dc.contributor.author	Young, P https://orcid.org/0000-0002-4357-7999	en_US
dc.date.available	2013-12-31	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Pharmaceutical Research, 2014, 31 (7), pp. 1779 - 1787	en_US
dc.identifier.issn	0724-8741	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115562
dc.description.abstract	Purpose: The purpose of this study was to present a modified Andersen cascade impactor (ACI) as a platform to evaluate the deposition and subsequent transport of aerosol micropaticles across airway epithelial cells. Methods: The impaction plate of an ACI was modified to accommodate up to eight Snapwells. Aerodynamic particle size distribution of the modified ACI was investigated with two commercially available formulations of Ventolin® (salbutamol sulphate) and QVAR® (beclomethasone dipropionate). Deposition and transport of these drug microparticles across sub-bronchial epithelial Calu-3 cells were also studied. Results: The modified ACI demonstrated reproducible deposition patterns of the commercially available pressurised metered dose inhalers compared to the standard ACI. Furthermore, the Calu-3 cells could be placed in different stages of the modified ACI. No significant effect was observed among the transport rate of different particle sizes deposited on Calu-3 cells within the range of 3.3 to 0.4 μm. Conclusions: The use of the cell compatible ACI to assess the fate of microparticles after deposition on the respiratory epithelia may allow for better understanding of deposited microparticles in vivo. © 2014 Springer Science+Business Media New York.	en_US
dc.relation.ispartof	Pharmaceutical Research	en_US
dc.relation.isbasedon	10.1007/s11095-013-1282-2	en_US
dc.subject.classification	Pharmacology & Pharmacy	en_US
dc.subject.mesh	Bronchi	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Epithelial Cells	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Albuterol	en_US
dc.subject.mesh	Beclomethasone	en_US
dc.subject.mesh	Anti-Asthmatic Agents	en_US
dc.subject.mesh	Aerosols	en_US
dc.subject.mesh	Administration, Inhalation	en_US
dc.subject.mesh	Metered Dose Inhalers	en_US
dc.title	In vitro cell integrated impactor deposition methodology for the study of aerodynamically relevant size fractions from commercial pressurised metered dose inhalers	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	31	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	31	en_US

Abstract:

Purpose: The purpose of this study was to present a modified Andersen cascade impactor (ACI) as a platform to evaluate the deposition and subsequent transport of aerosol micropaticles across airway epithelial cells. Methods: The impaction plate of an ACI was modified to accommodate up to eight Snapwells. Aerodynamic particle size distribution of the modified ACI was investigated with two commercially available formulations of Ventolin® (salbutamol sulphate) and QVAR® (beclomethasone dipropionate). Deposition and transport of these drug microparticles across sub-bronchial epithelial Calu-3 cells were also studied. Results: The modified ACI demonstrated reproducible deposition patterns of the commercially available pressurised metered dose inhalers compared to the standard ACI. Furthermore, the Calu-3 cells could be placed in different stages of the modified ACI. No significant effect was observed among the transport rate of different particle sizes deposited on Calu-3 cells within the range of 3.3 to 0.4 μm. Conclusions: The use of the cell compatible ACI to assess the fate of microparticles after deposition on the respiratory epithelia may allow for better understanding of deposited microparticles in vivo. © 2014 Springer Science+Business Media New York.

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http://hdl.handle.net/10453/115562