Single-cell states in the estrogen response of breast cancer cell lines

Casale, FP; Giurato, G; Nassa, G; Armond, JW; Oates, CJ; Corá, D; Gamba, A; Mukherjee, S; Weisz, A; Nicodemi, M

Single-cell states in the estrogen response of breast cancer cell lines

Casale, FP Giurato, G Nassa, G Armond, JW Oates, CJ

Corá, D Gamba, A Mukherjee, S Weisz, A Nicodemi, M

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2014, 9 (2)
Issue Date:: 2014-02-25

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Field	Value	Language
dc.contributor.author	Casale, FP	en_US
dc.contributor.author	Giurato, G	en_US
dc.contributor.author	Nassa, G	en_US
dc.contributor.author	Armond, JW	en_US
dc.contributor.author	Oates, CJ https://orcid.org/0000-0002-4444-8603	en_US
dc.contributor.author	Corá, D	en_US
dc.contributor.author	Gamba, A	en_US
dc.contributor.author	Mukherjee, S	en_US
dc.contributor.author	Weisz, A	en_US
dc.contributor.author	Nicodemi, M	en_US
dc.date.available	2014-01-07	en_US
dc.date.issued	2014-02-25	en_US
dc.identifier.citation	PLoS ONE, 2014, 9 (2)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115864
dc.description.abstract	Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified. © 2014 Casale et al.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0088485	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Breast Neoplasms	en_US
dc.subject.mesh	Estrogens	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Cell Cycle	en_US
dc.subject.mesh	Gene Expression Regulation, Neoplastic	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Transcriptome	en_US
dc.subject.mesh	MCF-7 Cells	en_US
dc.title	Single-cell states in the estrogen response of breast cancer cell lines	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	9	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to population averaged data. Here we obtain, for the first time, a characterization at the single-cell level of the states and expression signatures of a hormone-starved MCF-7 cell system responding to estrogen. To do so, we employ a recently proposed model that allows for dissecting single-cell states from time-course microarray data. We show that within 32 hours following stimulation, MCF-7 cells traverse, most likely, six states, with a faster early response followed by a progressive deceleration. We also derive the genome-wide transcriptional profiles of such single-cell states and their functional characterization. Our results support a scenario where estrogen promotes cell cycle progression by controlling multiple, sequential regulatory steps, whose single-cell events are here identified. © 2014 Casale et al.

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http://hdl.handle.net/10453/115864