Isolation and characterisation of cathepsin L1 protease from Fasciola hepatica excretory-secretory products for serodiagnosis of human fasciolosis
- Publisher:
- Humana Press Inc.
- Publication Type:
- Chapter
- Citation:
- Methods in Biotechnology: Food Borne Pathogens, 2006, 1, pp. 191 - 202
- Issue Date:
- 2006-01
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
![]() | 2005002874OK.pdf | 277.01 kB |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
The major antigens secreted by the parasite Fasciola hepatica are cathepsin-L cysteine proteases. The enzymes can be isolated from the parasite excretory-secretory products in sufficient quantities for use as an antigen for the serodiagnosis of humna fasciolosis. The methods illustrated in this chapter willexplain the isolation of cysteine proteases from E hepatica excretory-secretory products by gel filtration and anion exchange chromatography, and their subsequent characterisation and employment in an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-fasciola antibodies int he serum of infected humans
Please use this identifier to cite or link to this item: