An integrative study of a meromictic lake ecosystem in Antarctica

Lauro, FM; Demaere, MZ; Yau, S; Brown, MV; Ng, C; Wilkins, D; Raftery, MJ; Gibson, JA; Andrews-Pfannkoch, C; Lewis, M; Hoffman, JM; Thomas, T; Cavicchioli, R

An integrative study of a meromictic lake ecosystem in Antarctica

Lauro, FM Demaere, MZ

Yau, S Brown, MV Ng, C Wilkins, D Raftery, MJ Gibson, JA Andrews-Pfannkoch, C Lewis, M Hoffman, JM Thomas, T Cavicchioli, R

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Publication Type:: Journal Article
Citation:: ISME Journal, 2011, 5 (5), pp. 879 - 895
Issue Date:: 2011-05-01

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Field	Value	Language
dc.contributor.author	Lauro, FM	en_US
dc.contributor.author	Demaere, MZ https://orcid.org/0000-0002-7601-5108	en_US
dc.contributor.author	Yau, S	en_US
dc.contributor.author	Brown, MV	en_US
dc.contributor.author	Ng, C	en_US
dc.contributor.author	Wilkins, D	en_US
dc.contributor.author	Raftery, MJ	en_US
dc.contributor.author	Gibson, JA	en_US
dc.contributor.author	Andrews-Pfannkoch, C	en_US
dc.contributor.author	Lewis, M	en_US
dc.contributor.author	Hoffman, JM	en_US
dc.contributor.author	Thomas, T	en_US
dc.contributor.author	Cavicchioli, R	en_US
dc.date.issued	2011-05-01	en_US
dc.identifier.citation	ISME Journal, 2011, 5 (5), pp. 879 - 895	en_US
dc.identifier.issn	1751-7362	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117797
dc.description.abstract	In nature, the complexity and structure of microbial communities varies widely, ranging from a few species to thousands of species, and from highly structured to highly unstructured communities. Here, we describe the identity and functional capacity of microbial populations within distinct layers of a pristine, marine-derived, meromictic (stratified) lake (Ace Lake) in Antarctica. Nine million open reading frames were analyzed, representing microbial samples taken from six depths of the lake size fractionated on sequential 3.0, 0.8 and 0.1 m filters, and including metaproteome data from matching 0.1 m filters. We determine how the interactions of members of this highly structured and moderately complex community define the biogeochemical fluxes throughout the entire lake. Our view is that the health of this delicate ecosystem is dictated by the effects of the polar light cycle on the dominant role of green sulfur bacteria in primary production and nutrient cycling, and the influence of viruses/phage and phage resistance on the cooperation between members of the microbial community right throughout the lake. To test our assertions, and develop a framework applicable to other microbially driven ecosystems, we developed a mathematical model that describes how cooperation within a microbial system is impacted by periodic fluctuations in environmental parameters on key populations of microorganisms. Our study reveals a mutualistic structure within the microbial community throughout the lake that has arisen as the result of mechanistic interactions between the physico-chemical parameters and the selection of individual members of the community. By exhaustively describing and modelling interactions in Ace Lake, we have developed an approach that may be applicable to learning how environmental perturbations affect the microbial dynamics in more complex aquatic systems. © 2011 International Society for Microbial Ecology All rights reserved.	en_US
dc.relation.ispartof	ISME Journal	en_US
dc.relation.isbasedon	10.1038/ismej.2010.185	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Chlorobi	en_US
dc.subject.mesh	Viruses	en_US
dc.subject.mesh	Proteome	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Seasons	en_US
dc.subject.mesh	Fresh Water	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Open Reading Frames	en_US
dc.subject.mesh	Models, Biological	en_US
dc.subject.mesh	Antarctic Regions	en_US
dc.subject.mesh	Microbial Interactions	en_US
dc.title	An integrative study of a meromictic lake ecosystem in Antarctica	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.for	060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)	en_US
utslib.for	050205 Environmental Management	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

In nature, the complexity and structure of microbial communities varies widely, ranging from a few species to thousands of species, and from highly structured to highly unstructured communities. Here, we describe the identity and functional capacity of microbial populations within distinct layers of a pristine, marine-derived, meromictic (stratified) lake (Ace Lake) in Antarctica. Nine million open reading frames were analyzed, representing microbial samples taken from six depths of the lake size fractionated on sequential 3.0, 0.8 and 0.1 m filters, and including metaproteome data from matching 0.1 m filters. We determine how the interactions of members of this highly structured and moderately complex community define the biogeochemical fluxes throughout the entire lake. Our view is that the health of this delicate ecosystem is dictated by the effects of the polar light cycle on the dominant role of green sulfur bacteria in primary production and nutrient cycling, and the influence of viruses/phage and phage resistance on the cooperation between members of the microbial community right throughout the lake. To test our assertions, and develop a framework applicable to other microbially driven ecosystems, we developed a mathematical model that describes how cooperation within a microbial system is impacted by periodic fluctuations in environmental parameters on key populations of microorganisms. Our study reveals a mutualistic structure within the microbial community throughout the lake that has arisen as the result of mechanistic interactions between the physico-chemical parameters and the selection of individual members of the community. By exhaustively describing and modelling interactions in Ace Lake, we have developed an approach that may be applicable to learning how environmental perturbations affect the microbial dynamics in more complex aquatic systems. © 2011 International Society for Microbial Ecology All rights reserved.

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http://hdl.handle.net/10453/117797