Osteogenic potential of mouse adipose-derived stem cells sorted for CD90 and CD105 in Vitro

Yamamoto, M; Nakata, H; Hao, J; Chou, J; Kasugai, S; Kuroda, S

Osteogenic potential of mouse adipose-derived stem cells sorted for CD90 and CD105 in Vitro

Yamamoto, M Nakata, H Hao, J Chou, J

Kasugai, S Kuroda, S

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Publication Type:: Journal Article
Citation:: Stem Cells International, 2014, 2014
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Yamamoto, M	en_US
dc.contributor.author	Nakata, H	en_US
dc.contributor.author	Hao, J	en_US
dc.contributor.author	Chou, J https://orcid.org/0000-0002-7472-8016	en_US
dc.contributor.author	Kasugai, S	en_US
dc.contributor.author	Kuroda, S	en_US
dc.date.available	2014-08-12	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Stem Cells International, 2014, 2014	en_US
dc.identifier.uri	http://hdl.handle.net/10453/120311
dc.description.abstract	© 2014 Maiko Yamamoto et al. Adipose tissue-derived stromal cells, termed ASCs, play an important role in regenerative applications. They resemble mesenchymal stem cells owing to their inexhaustibility, general differentiation potential, and plasticity and display a series of cell-specific and cluster-of-differentiation (CD) marker profiles similar to those of other somatic stem cells. Variations in phenotypes or differentiation are intimately associated with CD markers. The purpose of our study was to exhibit distinct populations of ASCs with differing characteristics for osteogenic differentiation. The primary cell batch of murine-derived ASCs was extracted from subcutaneous adipose tissue and the cells were sorted for the expression of the surface protein molecules CD90 and CD105 using flow cytometry. Each cell population sorted for CD90 and CD105 was analyzed for osteogenic potency after cell culture. The results suggested that ASCs exhibit distinct populations with differing characteristics for osteogenic differentiation: unsorted ASCs stimulated comparable mineralized nodule formation as bone marrow stromal cells (BMSCs) in osteogenic medium and viral transfection for BMP2 accelerated the formation of mineralized nodules in CD90 and/or CD105 positive ASCs with observation of decrease in CD105 expression after 14 days. Future studies assessing different immunophenotypes of ASCs should be undertaken to develop cell-based tissue engineering.	en_US
dc.relation.ispartof	Stem Cells International	en_US
dc.relation.isbasedon	10.1155/2014/576358	en_US
dc.title	Osteogenic potential of mouse adipose-derived stem cells sorted for CD90 and CD105 in Vitro	en_US
dc.type	Journal Article
utslib.citation.volume	2014	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	2014	en_US

Abstract:

© 2014 Maiko Yamamoto et al. Adipose tissue-derived stromal cells, termed ASCs, play an important role in regenerative applications. They resemble mesenchymal stem cells owing to their inexhaustibility, general differentiation potential, and plasticity and display a series of cell-specific and cluster-of-differentiation (CD) marker profiles similar to those of other somatic stem cells. Variations in phenotypes or differentiation are intimately associated with CD markers. The purpose of our study was to exhibit distinct populations of ASCs with differing characteristics for osteogenic differentiation. The primary cell batch of murine-derived ASCs was extracted from subcutaneous adipose tissue and the cells were sorted for the expression of the surface protein molecules CD90 and CD105 using flow cytometry. Each cell population sorted for CD90 and CD105 was analyzed for osteogenic potency after cell culture. The results suggested that ASCs exhibit distinct populations with differing characteristics for osteogenic differentiation: unsorted ASCs stimulated comparable mineralized nodule formation as bone marrow stromal cells (BMSCs) in osteogenic medium and viral transfection for BMP2 accelerated the formation of mineralized nodules in CD90 and/or CD105 positive ASCs with observation of decrease in CD105 expression after 14 days. Future studies assessing different immunophenotypes of ASCs should be undertaken to develop cell-based tissue engineering.

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http://hdl.handle.net/10453/120311