The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model

Ramakrishnan, C; Walker, RA; Eichenberger, RM; Hehl, AB; Smith, NC

The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model

Ramakrishnan, C Walker, RA Eichenberger, RM Hehl, AB Smith, NC

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2017, 47 (10-11), pp. 597 - 600
Issue Date:: 2017-09-01

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Field	Value	Language
dc.contributor.author	Ramakrishnan, C	en_US
dc.contributor.author	Walker, RA	en_US
dc.contributor.author	Eichenberger, RM	en_US
dc.contributor.author	Hehl, AB	en_US
dc.contributor.author	Smith, NC https://orcid.org/0000-0002-7467-1451	en_US
dc.date.available	2017-04-11	en_US
dc.date.issued	2017-09-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2017, 47 (10-11), pp. 597 - 600	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125277
dc.description.abstract	© 2017 Australian Society for Parasitology The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/j.ijpara.2017.04.001	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Intestines	en_US
dc.subject.mesh	Vacuoles	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cats	en_US
dc.subject.mesh	Toxoplasma	en_US
dc.subject.mesh	Toxoplasmosis, Animal	en_US
dc.subject.mesh	Cat Diseases	en_US
dc.subject.mesh	Protozoan Proteins	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	Protein Conformation	en_US
dc.subject.mesh	Merozoites	en_US
dc.title	The merozoite-specific protein, TgGRA11B, identified as a component of the Toxoplasma gondii parasitophorous vacuole in a tachyzoite expression model	en_US
dc.type	Journal Article
utslib.citation.volume	10-11	en_US
utslib.citation.volume	47	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	10-11	en_US
pubs.publication-status	Published	en_US
pubs.volume	47	en_US

Abstract:

© 2017 Australian Society for Parasitology The apicomplexan, Toxoplasma gondii, infects all warm-blooded animals as intermediate hosts but only felids as definitive hosts. Dense granule proteins are critical for the survival of Toxoplasma within host cells but, whilst these proteins have been studied intensively in tachyzoites, little is known about their expression in the coccidian stages in the cat intestine. Transcriptomic profiling indicates that two putative dense granule proteins, TgGRA11A and TgGRA11B, are expressed uniquely in merozoites. Immunofluorescent microscopy of Toxoplasma-infected cat intestine and tachyzoites engineered to express TgGRA11B, reveals that it is a dense granule protein that traffics into the parasitophorous vacuole and its membrane.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/125277