Conservation of proteins involved in oocyst wall formation in Eimeria maxima, Eimeria tenella and Eimeria acervulina

Belli, SI; Ferguson, DJP; Katrib, M; Slapetova, I; Mai, K; Slapeta, J; Flowers, SA; Miska, KB; Tomley, FM; Shirley, MW; Wallach, MG; Smith, NC

Conservation of proteins involved in oocyst wall formation in Eimeria maxima, Eimeria tenella and Eimeria acervulina

Belli, SI

Ferguson, DJP Katrib, M Slapetova, I Mai, K Slapeta, J

Flowers, SA

Miska, KB Tomley, FM Shirley, MW Wallach, MG Smith, NC

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2009, 39 (10), pp. 1063 - 1070
Issue Date:: 2009-08-01

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Field	Value	Language
dc.contributor.author	Belli, SI https://orcid.org/0000-0003-3845-326X	en_US
dc.contributor.author	Ferguson, DJP	en_US
dc.contributor.author	Katrib, M	en_US
dc.contributor.author	Slapetova, I	en_US
dc.contributor.author	Mai, K	en_US
dc.contributor.author	Slapeta, J https://orcid.org/0000-0003-1459-9117	en_US
dc.contributor.author	Flowers, SA https://orcid.org/0000-0002-3513-2260	en_US
dc.contributor.author	Miska, KB	en_US
dc.contributor.author	Tomley, FM	en_US
dc.contributor.author	Shirley, MW	en_US
dc.contributor.author	Wallach, MG	en_US
dc.contributor.author	Smith, NC https://orcid.org/0000-0002-7467-1451	en_US
dc.date.available	2009-05-18	en_US
dc.date.issued	2009-08-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2009, 39 (10), pp. 1063 - 1070	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/12781
dc.description.abstract	Vaccination with proteins from gametocytes of Eimeria maxima protects chickens, via transfer of maternal antibodies, against infection with several species of Eimeria. Antibodies to E. maxima gametocyte proteins recognise proteins in the wall forming bodies of macrogametocytes and oocyst walls of E. maxima, Eimeria tenella and Eimeria acervulina. Homologous genes for two major gametocyte proteins - GAM56 and GAM82 - were found in E. maxima, E. tenella and E. acervulina. Alignment of the predicted protein sequences of these genes reveals that, as well as sharing regions of tyrosine richness, strong homology exists in their amino-terminal regions, where protective antibodies bind. This study confirms the conservation of the roles of GAM56 and GAM82 in oocyst wall formation and shows that antibodies to gametocyte antigens of E. maxima cross-react with homologous proteins in other species, helping to explain cross-species maternal immunity. © 2009 Australian Society for Parasitology Inc.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/j.ijpara.2009.05.004	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Chickens	en_US
dc.subject.mesh	Eimeria	en_US
dc.subject.mesh	Eimeria tenella	en_US
dc.subject.mesh	Oocysts	en_US
dc.subject.mesh	Protozoan Proteins	en_US
dc.subject.mesh	DNA, Protozoan	en_US
dc.subject.mesh	Antigens, Protozoan	en_US
dc.subject.mesh	Fluorescent Antibody Technique, Indirect	en_US
dc.subject.mesh	Gene Expression Regulation, Developmental	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Conservation of proteins involved in oocyst wall formation in Eimeria maxima, Eimeria tenella and Eimeria acervulina	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	39	en_US
utslib.for	0707 Veterinary Sciences	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	39	en_US

Abstract:

Vaccination with proteins from gametocytes of Eimeria maxima protects chickens, via transfer of maternal antibodies, against infection with several species of Eimeria. Antibodies to E. maxima gametocyte proteins recognise proteins in the wall forming bodies of macrogametocytes and oocyst walls of E. maxima, Eimeria tenella and Eimeria acervulina. Homologous genes for two major gametocyte proteins - GAM56 and GAM82 - were found in E. maxima, E. tenella and E. acervulina. Alignment of the predicted protein sequences of these genes reveals that, as well as sharing regions of tyrosine richness, strong homology exists in their amino-terminal regions, where protective antibodies bind. This study confirms the conservation of the roles of GAM56 and GAM82 in oocyst wall formation and shows that antibodies to gametocyte antigens of E. maxima cross-react with homologous proteins in other species, helping to explain cross-species maternal immunity. © 2009 Australian Society for Parasitology Inc.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/12781