Cinnamaldehyde disrupts biofilm formation and swarming motility of pseudomonas aeruginosa

Topa, SH; Subramoni, S; Palombo, EA; Kingshott, P; Rice, SA; Blackall, LL

Cinnamaldehyde disrupts biofilm formation and swarming motility of pseudomonas aeruginosa

Topa, SH Subramoni, S Palombo, EA Kingshott, P Rice, SA

Blackall, LL

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Publication Type:: Journal Article
Citation:: Microbiology (United Kingdom), 2018, 164 (9), pp. 1087 - 1097
Issue Date:: 2018-09-01

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Field	Value	Language
dc.contributor.author	Topa, SH	en_US
dc.contributor.author	Subramoni, S	en_US
dc.contributor.author	Palombo, EA	en_US
dc.contributor.author	Kingshott, P	en_US
dc.contributor.author	Rice, SA https://orcid.org/0000-0002-9486-2343	en_US
dc.contributor.author	Blackall, LL	en_US
dc.date.issued	2018-09-01	en_US
dc.identifier.citation	Microbiology (United Kingdom), 2018, 164 (9), pp. 1087 - 1097	en_US
dc.identifier.issn	1350-0872	en_US
dc.identifier.uri	http://hdl.handle.net/10453/127857
dc.description.abstract	© 2018 The Authors. Bacterial biofilms can cause serious health care complications associated with increased morbidity and mortality. There is an urge to discover and develop new biofilm inhibitors from natural products or by modifying natural compounds or understanding the modes of action of existing compounds. Cinnamaldehyde (CAD), one of the major components of cinnamon oil, has been demonstrated to act as an antimicrobial agent against a number of Gram-negative and Gram-positive pathogens, including Pseudomonas aeruginosa, Helicobacter pylori and Listeria monocytogenes. Despite the mechanism of action of CAD against the model organism P. aeruginosa being undefined, based on its antimicrobial properties, we hypothesized that it may disrupt preformed biofilms of P. aeruginosa. The minimum inhibitory concentration (MIC) of CAD for planktonic P. aeruginosa was determined to be 11.8 mM. Membrane depolarization assays demonstrated disruption of the transmembrane potential of P. aeruginosa. CAD at 5.9mM (0.5 MIC) disrupted preformed biofilms by 75.6% and 3mM CAD (0.25 MIC) reduced the intracellular concentrations of the secondary messenger, bis-(3′–5′)-cyclic dimeric guanosine monophosphate (c-di-GMP), which controls P. aeruginosa biofilm formation. The swarming motility of P. aeruginosa was also reduced by CAD in a concentration-dependent manner. Collectively, these findings show that sub-MICs of CAD can disrupt biofilms and other surface colonization phenotypes through the modulation of intracellular signalling processes.	en_US
dc.relation.ispartof	Microbiology (United Kingdom)	en_US
dc.relation.isbasedon	10.1099/mic.0.000692	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Acrolein	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Microbial Sensitivity Tests	en_US
dc.subject.mesh	Membrane Potentials	en_US
dc.subject.mesh	Locomotion	en_US
dc.subject.mesh	Secondary Metabolism	en_US
dc.title	Cinnamaldehyde disrupts biofilm formation and swarming motility of pseudomonas aeruginosa	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	164	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	164	en_US

Abstract:

© 2018 The Authors. Bacterial biofilms can cause serious health care complications associated with increased morbidity and mortality. There is an urge to discover and develop new biofilm inhibitors from natural products or by modifying natural compounds or understanding the modes of action of existing compounds. Cinnamaldehyde (CAD), one of the major components of cinnamon oil, has been demonstrated to act as an antimicrobial agent against a number of Gram-negative and Gram-positive pathogens, including Pseudomonas aeruginosa, Helicobacter pylori and Listeria monocytogenes. Despite the mechanism of action of CAD against the model organism P. aeruginosa being undefined, based on its antimicrobial properties, we hypothesized that it may disrupt preformed biofilms of P. aeruginosa. The minimum inhibitory concentration (MIC) of CAD for planktonic P. aeruginosa was determined to be 11.8 mM. Membrane depolarization assays demonstrated disruption of the transmembrane potential of P. aeruginosa. CAD at 5.9mM (0.5 MIC) disrupted preformed biofilms by 75.6% and 3mM CAD (0.25 MIC) reduced the intracellular concentrations of the secondary messenger, bis-(3′–5′)-cyclic dimeric guanosine monophosphate (c-di-GMP), which controls P. aeruginosa biofilm formation. The swarming motility of P. aeruginosa was also reduced by CAD in a concentration-dependent manner. Collectively, these findings show that sub-MICs of CAD can disrupt biofilms and other surface colonization phenotypes through the modulation of intracellular signalling processes.

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http://hdl.handle.net/10453/127857