Nuclear staining of telogen hair roots contributes to successful forensic nDNA analysis

Brooks, EM; Cullen, M; Sztydna, T; Walsh, SJ

Nuclear staining of telogen hair roots contributes to successful forensic nDNA analysis

Brooks, EM Cullen, M Sztydna, T

Walsh, SJ

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Publication Type:: Journal Article
Citation:: Australian Journal of Forensic Sciences, 2010, 42 (2), pp. 115 - 122
Issue Date:: 2010-06-01

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Field	Value	Language
dc.contributor.author	Brooks, EM	en_US
dc.contributor.author	Cullen, M	en_US
dc.contributor.author	Sztydna, T https://orcid.org/0000-0002-8957-0864	en_US
dc.contributor.author	Walsh, SJ	en_US
dc.date.issued	2010-06-01	en_US
dc.identifier.citation	Australian Journal of Forensic Sciences, 2010, 42 (2), pp. 115 - 122	en_US
dc.identifier.issn	0045-0618	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13077
dc.description.abstract	Telogen hairs have generally presented significant challenges to routine and advanced forensic nDNA typing techniques. Nuclear specific DAPI fluorescence staining of telogen hair roots introduced a method of determining the number of nuclei still present in the hair root. However, a simple fixation step has increased the yield of complete nDNA profiles over the unfixed telogen root and the introduction of Harris' Haematoxylin nuclear specific staining makes this technique readily available to all forensic biology laboratories. © 2010 Australian Academy of Forensic Sciences.	en_US
dc.relation.ispartof	Australian Journal of Forensic Sciences	en_US
dc.relation.isbasedon	10.1080/00450610903258136	en_US
dc.subject.classification	Legal & Forensic Medicine	en_US
dc.title	Nuclear staining of telogen hair roots contributes to successful forensic nDNA analysis	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	42	en_US
utslib.for	0399 Other Chemical Sciences	en_US
dc.location.activity	ISI:000278010200004	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	42	en_US

Abstract:

Telogen hairs have generally presented significant challenges to routine and advanced forensic nDNA typing techniques. Nuclear specific DAPI fluorescence staining of telogen hair roots introduced a method of determining the number of nuclei still present in the hair root. However, a simple fixation step has increased the yield of complete nDNA profiles over the unfixed telogen root and the introduction of Harris' Haematoxylin nuclear specific staining makes this technique readily available to all forensic biology laboratories. © 2010 Australian Academy of Forensic Sciences.

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http://hdl.handle.net/10453/13077