A new system for three-dimensional tracking of motile microorganisms

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Journal Article
Applied and Environmental Microbiology, 2000, 66 (5), pp. 2238 - 2242
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A new three-dimensional (3D)-tracking system with optimized dark-field illumination is presented. It allows simultaneous 3D tracking of several free-swimming microorganisms with diameters of > 10 μm. Resolution limits and illumination efficiencies for different size classes of microorganisms are treated analytically. First applications for 3D tracking of protists are demonstrated.
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