Matching sensitivity to abundance: High resolution immuno-mass spectrometry imaging of lanthanide labels and endogenous elements in the murine brain

Clases, D; Gonzalez De Vega, R; Funke, S; Lockwood, TE; Westerhausen, MT; Taudte, RV; Adlard, PA; Doble, PA

Matching sensitivity to abundance: High resolution immuno-mass spectrometry imaging of lanthanide labels and endogenous elements in the murine brain

Clases, D

Gonzalez De Vega, R Funke, S Lockwood, TE Westerhausen, MT Taudte, RV Adlard, PA Doble, PA

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Journal of Analytical Atomic Spectrometry, 2020, 35, (4), pp. 728-735
Issue Date:: 2020-04-01

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Field	Value	Language
dc.contributor.author	Clases, D https://orcid.org/0000-0003-3880-9385
dc.contributor.author	Gonzalez De Vega, R
dc.contributor.author	Funke, S
dc.contributor.author	Lockwood, TE
dc.contributor.author	Westerhausen, MT
dc.contributor.author	Taudte, RV
dc.contributor.author	Adlard, PA
dc.contributor.author	Doble, PA
dc.date.accessioned	2020-12-18T01:24:42Z
dc.date.available	2020-12-18T01:24:42Z
dc.date.issued	2020-04-01
dc.identifier.citation	Journal of Analytical Atomic Spectrometry, 2020, 35, (4), pp. 728-735
dc.identifier.issn	0267-9477
dc.identifier.issn	1364-5544
dc.identifier.uri	http://hdl.handle.net/10453/144829
dc.description.abstract	© 2020 The Royal Society of Chemistry. This work introduces a new method for immuno-mass spectrometry imaging via quadrupole-based laser ablation-inductively coupled plasma-mass spectrometry instruments that is matched to the abundance of elements in biological tissues. Manipulation of ion-optics and quadrupole mass filter parameters provided increased transmission of low level high-mass elements, which are typically used as labels for antibodies, at the expense of highly abundant endogenous low-mass elements. Transmission of mid-mass elements such as transition metals was only slightly affected. The implications for mass resolution and background signals are critically discussed and signal to noise ratios and imaging capabilities are compared to those obtained from a standard method. This novel approach resulted in a 6-fold improved signal to noise ratio for lanthanides that are routinely used as elemental labels for antibodies to target protein distributions in biological tissues. This increase in signal intensity, enhanced contrasts, lower limits of detection and the potential to improve spatial resolution contributed to enhanced imaging and trace analyses, as demonstrated by imaging murine brain sections of the hippocampal system and substantia nigra.
dc.language	English
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation	http://purl.org/au-research/grants/arc/DP170100036
dc.relation	German Research Foundation417283954
dc.relation.ispartof	Journal of Analytical Atomic Spectrometry
dc.relation.isbasedon	10.1039/c9ja00405j
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0301 Analytical Chemistry, 0306 Physical Chemistry (incl. Structural), 0403 Geology
dc.subject.classification	Analytical Chemistry
dc.title	Matching sensitivity to abundance: High resolution immuno-mass spectrometry imaging of lanthanide labels and endogenous elements in the murine brain
dc.type	Journal Article
utslib.citation.volume	35
utslib.for	0301 Analytical Chemistry
utslib.for	0306 Physical Chemistry (incl. Structural)
utslib.for	0403 Geology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2020-12-18T01:24:29Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	35
utslib.citation.issue	4

Abstract:

© 2020 The Royal Society of Chemistry. This work introduces a new method for immuno-mass spectrometry imaging via quadrupole-based laser ablation-inductively coupled plasma-mass spectrometry instruments that is matched to the abundance of elements in biological tissues. Manipulation of ion-optics and quadrupole mass filter parameters provided increased transmission of low level high-mass elements, which are typically used as labels for antibodies, at the expense of highly abundant endogenous low-mass elements. Transmission of mid-mass elements such as transition metals was only slightly affected. The implications for mass resolution and background signals are critically discussed and signal to noise ratios and imaging capabilities are compared to those obtained from a standard method. This novel approach resulted in a 6-fold improved signal to noise ratio for lanthanides that are routinely used as elemental labels for antibodies to target protein distributions in biological tissues. This increase in signal intensity, enhanced contrasts, lower limits of detection and the potential to improve spatial resolution contributed to enhanced imaging and trace analyses, as demonstrated by imaging murine brain sections of the hippocampal system and substantia nigra.

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http://hdl.handle.net/10453/144829