Determination of lysozyme using microcantilever sensor based on atomic force microscopy

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Journal Article
Fenxi Huaxue/ Chinese Journal of Analytical Chemistry, 2010, 38 (12), pp. 1771 - 1775
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A microcantilever sensor was developed to detect lysozyme in a pharmaceutical formulation based on the principle of atomic force microscopy. By measuring the cantilever bending and deflection using the optical reflection technique, the lysozyme adsorption on a dodecanethiol-modified cantilever surface was detected. Under optimal conditions, a good linear relationship between the deflection of the microcantilever and the logarithm of the lysozyme concentration in the range from 10 ng L-1 to 0.1 mg L-1, with a correlation coefficient of 0.998, was established. The limit of detection was 5.0 ng L-1. The lysozyme in the pharmaceutical formulation sample was determined with the desirable results by using the present method. Copyright © 2010, Changchun Institute of Applied Chemistry.
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