Determination of Lysozyme Using Microcantilever Sensor Based on Atomic Force Microscopy

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Journal Article
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2010, 38 (12), pp. 1771 - 1775
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A novel sensor based on an atomic force microscopy (AFM) microcantilever was developed to detect lysozome in a pharmaceutical formulation sample. By measuring the cantilever bending, using the optical reflection technique, the lysozyme adsorption by a dodecanethiol-modified cantilever surface was detected in real time. Under optimal conditions, a linear relationship was established between the deflection of the cantilever and the logarithm of the concentration of lysozome in the concentration range of 0.01 - 100 mu g/L with a correlation coefficient of 0.998; the limit of detection(S/N = 3) was 5.0 ng/L. The method described has been applied to determine lysozyme in pharmaceutical formulation sample with satisfactory results.
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