Environmental bacteriophages active on biofilms and planktonic forms of toxigenic Vibrio cholerae: Potential relevance in cholera epidemiology.

Naser, IB; Hoque, MM; Abdullah, A; Bari, SMN; Ghosh, AN; Faruque, SM

Environmental bacteriophages active on biofilms and planktonic forms of toxigenic Vibrio cholerae: Potential relevance in cholera epidemiology.

Naser, IB Hoque, MM Abdullah, A Bari, SMN Ghosh, AN Faruque, SM

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Publisher:: PUBLIC LIBRARY SCIENCE
Publication Type:: Journal Article
Citation:: PLoS One, 2017, 12, (7), pp. e0180838
Issue Date:: 2017

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Field	Value	Language
dc.contributor.author	Naser, IB
dc.contributor.author	Hoque, MM
dc.contributor.author	Abdullah, A
dc.contributor.author	Bari, SMN
dc.contributor.author	Ghosh, AN
dc.contributor.author	Faruque, SM
dc.date.accessioned	2022-04-01T03:08:35Z
dc.date.available	2017-06-22
dc.date.available	2022-04-01T03:08:35Z
dc.date.issued	2017
dc.identifier.citation	PLoS One, 2017, 12, (7), pp. e0180838
dc.identifier.issn	1932-6203
dc.identifier.issn	1932-6203
dc.identifier.uri	http://hdl.handle.net/10453/155857
dc.description.abstract	METHODS: Phages isolated from environmental waters in Bangladesh were tested for their host specificity towards V. cholerae O1 and O139, and the ability to disperse V. cholerae biofilms formed in the laboratory. Representative phages were further characterized by electron microscopy and whole genome sequencing. Selected phages were then introduced in various combinations to biofilms of toxigenic V. cholerae added to samples of river water, and the dispersion of biofilms as well as the growth kinetics of V. cholerae and the phages were monitored. RESULTS: A phage cocktail composed of three different phages isolated from surface waters in Bangladesh and designated as JSF7, JSF4, and JSF3 could significantly influence the distribution and concentration of the active planktonic form and biofilm associated form of toxigenic V. cholerae in water. While JSF7 showed a biofilm degrading activity and dispersed cells from both V. cholerae O1 and O139 derived biofilms thus increasing the concentration of planktonic V. cholerae in water, JSF4 and JSF3 showed strong bactericidal activity against V. cholerae O1 and O139 respectively. A mixture of all three phages could effectively reduce both biofilm-associated and planktonic V. cholerae in river water microcosms. SIGNIFICANCE: Besides potential applicability in phage-mediated control of cholera, our results have relevance in appreciating possible intricate role of diverse environmental phages in the epidemiology of the disease, since both biofilms and phages influence the prevalence and infectivity of V. cholerae in a variety of ways.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	PUBLIC LIBRARY SCIENCE
dc.relation.ispartof	PLoS One
dc.relation.isbasedon	10.1371/journal.pone.0180838
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	General Science & Technology
dc.subject.mesh	Bacteriophages
dc.subject.mesh	Biofilms
dc.subject.mesh	Cholera
dc.subject.mesh	Plankton
dc.subject.mesh	Vibrio cholerae
dc.subject.mesh	Vibrio cholerae O1
dc.subject.mesh	Vibrio cholerae O139
dc.subject.mesh	Water Microbiology
dc.subject.mesh	Plankton
dc.subject.mesh	Biofilms
dc.subject.mesh	Vibrio cholerae
dc.subject.mesh	Vibrio cholerae O1
dc.subject.mesh	Vibrio cholerae O139
dc.subject.mesh	Bacteriophages
dc.subject.mesh	Cholera
dc.subject.mesh	Water Microbiology
dc.title	Environmental bacteriophages active on biofilms and planktonic forms of toxigenic Vibrio cholerae: Potential relevance in cholera epidemiology.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-04-01T03:08:11Z
pubs.issue	7
pubs.publication-status	Published online
pubs.volume	12
utslib.citation.issue	7

Abstract:

METHODS: Phages isolated from environmental waters in Bangladesh were tested for their host specificity towards V. cholerae O1 and O139, and the ability to disperse V. cholerae biofilms formed in the laboratory. Representative phages were further characterized by electron microscopy and whole genome sequencing. Selected phages were then introduced in various combinations to biofilms of toxigenic V. cholerae added to samples of river water, and the dispersion of biofilms as well as the growth kinetics of V. cholerae and the phages were monitored. RESULTS: A phage cocktail composed of three different phages isolated from surface waters in Bangladesh and designated as JSF7, JSF4, and JSF3 could significantly influence the distribution and concentration of the active planktonic form and biofilm associated form of toxigenic V. cholerae in water. While JSF7 showed a biofilm degrading activity and dispersed cells from both V. cholerae O1 and O139 derived biofilms thus increasing the concentration of planktonic V. cholerae in water, JSF4 and JSF3 showed strong bactericidal activity against V. cholerae O1 and O139 respectively. A mixture of all three phages could effectively reduce both biofilm-associated and planktonic V. cholerae in river water microcosms. SIGNIFICANCE: Besides potential applicability in phage-mediated control of cholera, our results have relevance in appreciating possible intricate role of diverse environmental phages in the epidemiology of the disease, since both biofilms and phages influence the prevalence and infectivity of V. cholerae in a variety of ways.

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http://hdl.handle.net/10453/155857