Characterization and in vitro probiotic assessment of potential indigenous Bacillus strains isolated from soil rhizosphere

Mohkam, M; Rasoul-Amini, S; Shokri, D; Berenjian, A; Rahimi, F; Sadraeian, M; Khalvati, B; Gholami, A; Ghasemi, Y

Characterization and in vitro probiotic assessment of potential indigenous Bacillus strains isolated from soil rhizosphere

Mohkam, M Rasoul-Amini, S Shokri, D Berenjian, A Rahimi, F Sadraeian, M

Khalvati, B Gholami, A Ghasemi, Y

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Publisher:: EDIZIONI MINERVA MEDICA
Publication Type:: Journal Article
Citation:: Minerva Biotecnologica, 2016, 28, (1), pp. 19-28
Issue Date:: 2016-03-01

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Field	Value	Language
dc.contributor.author	Mohkam, M
dc.contributor.author	Rasoul-Amini, S
dc.contributor.author	Shokri, D
dc.contributor.author	Berenjian, A
dc.contributor.author	Rahimi, F
dc.contributor.author	Sadraeian, M https://orcid.org/0000-0002-7384-2247
dc.contributor.author	Khalvati, B
dc.contributor.author	Gholami, A
dc.contributor.author	Ghasemi, Y
dc.date.accessioned	2022-08-16T01:03:27Z
dc.date.available	2022-08-16T01:03:27Z
dc.date.issued	2016-03-01
dc.identifier.citation	Minerva Biotecnologica, 2016, 28, (1), pp. 19-28
dc.identifier.issn	1120-4826
dc.identifier.issn	1827-160X
dc.identifier.uri	http://hdl.handle.net/10453/160287
dc.description.abstract	BACKGROUND: Probiotics mainly Bacillus species can be advantageous to the host by promoting its intestinal balance. Attempts were made to isolate and identify Bacillus strains from rhizosphere environment. METHODS: The in vitro probiotic criteria were used for screening and characterizing potential Bacillus probiotics. Morphological, physiological and biochemical characteristics as well as 16S rRNA gene sequence analysis were utilized for identification of the isolates. Seven isolates were chosen based on withstanding to acidic condition (pH 2.5) and various bile salt concentrations (1-4%(w/v)). RESULTS: Isolates found to have the least antimicrobial activity against Listeria monocytogenes PTCC 1163, Staphylococcus aureus ATCC 1912 and Bacillus cereus PTCC 1015; however, no activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 25922 was observed. The Bacillus Isolates showed different variation in auto-aggregation features and adhesion to hydrocarbons ranging from 60% to 90% and 10% to 60%, respectively. Excluding isolate 14 that exhibited resistance to penicillin and ampicillin, all the other Bacillus strains were sensitive to the tested antibiotics. All isolates showed relatively low cytotoxic effect on HepG2 cell line except strains 12 and 14. CONCLUSION: Taking together, among the investigated Bacillus isolates, strains 17 and S10 found to be the most promising candidates to fulfill in vitro probiotic specifications.
dc.language	English
dc.publisher	EDIZIONI MINERVA MEDICA
dc.relation.ispartof	Minerva Biotecnologica
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 10 Technology
dc.subject.classification	Biotechnology
dc.title	Characterization and in vitro probiotic assessment of potential indigenous Bacillus strains isolated from soil rhizosphere
dc.type	Journal Article
utslib.citation.volume	28
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-08-16T01:03:23Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	28
utslib.citation.issue	1

Abstract:

BACKGROUND: Probiotics mainly Bacillus species can be advantageous to the host by promoting its intestinal balance. Attempts were made to isolate and identify Bacillus strains from rhizosphere environment. METHODS: The in vitro probiotic criteria were used for screening and characterizing potential Bacillus probiotics. Morphological, physiological and biochemical characteristics as well as 16S rRNA gene sequence analysis were utilized for identification of the isolates. Seven isolates were chosen based on withstanding to acidic condition (pH 2.5) and various bile salt concentrations (1-4%(w/v)). RESULTS: Isolates found to have the least antimicrobial activity against Listeria monocytogenes PTCC 1163, Staphylococcus aureus ATCC 1912 and Bacillus cereus PTCC 1015; however, no activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 25922 was observed. The Bacillus Isolates showed different variation in auto-aggregation features and adhesion to hydrocarbons ranging from 60% to 90% and 10% to 60%, respectively. Excluding isolate 14 that exhibited resistance to penicillin and ampicillin, all the other Bacillus strains were sensitive to the tested antibiotics. All isolates showed relatively low cytotoxic effect on HepG2 cell line except strains 12 and 14. CONCLUSION: Taking together, among the investigated Bacillus isolates, strains 17 and S10 found to be the most promising candidates to fulfill in vitro probiotic specifications.

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http://hdl.handle.net/10453/160287