The HLDA8 blind panel: Findings and conclusions
Swart, B
Salganik, MP
Wand, MP
Tinckam, K
Milford, EL
Drbal, K
Angelisova, P
Horejsi, V
MacArdle, P
Bailey, S
Hollemweguer, E
Hodge, G
Nairn, J
Millard, D
Dagdeviren, A
Dandie, GW
Zola, H
- Publication Type:
- Journal Article
- Citation:
- Journal of Immunological Methods, 2005, 305 (1), pp. 75 - 83
- Issue Date:
- 2005-10-20
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
2010005202OK.pdf | 1.53 MB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Swart, B | en_US |
dc.contributor.author | Salganik, MP | en_US |
dc.contributor.author |
Wand, MP https://orcid.org/0000-0003-2555-896X |
en_US |
dc.contributor.author | Tinckam, K | en_US |
dc.contributor.author | Milford, EL | en_US |
dc.contributor.author | Drbal, K | en_US |
dc.contributor.author | Angelisova, P | en_US |
dc.contributor.author | Horejsi, V | en_US |
dc.contributor.author | MacArdle, P | en_US |
dc.contributor.author | Bailey, S | en_US |
dc.contributor.author | Hollemweguer, E | en_US |
dc.contributor.author | Hodge, G | en_US |
dc.contributor.author | Nairn, J | en_US |
dc.contributor.author | Millard, D | en_US |
dc.contributor.author | Dagdeviren, A | en_US |
dc.contributor.author | Dandie, GW | en_US |
dc.contributor.author | Zola, H | en_US |
dc.date.available | 2005-06-03 | en_US |
dc.date.issued | 2005-10-20 | en_US |
dc.identifier.citation | Journal of Immunological Methods, 2005, 305 (1), pp. 75 - 83 | en_US |
dc.identifier.issn | 0022-1759 | en_US |
dc.identifier.uri | http://hdl.handle.net/10453/17008 | |
dc.description.abstract | There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. © 2005 Elsevier B.V. All rights reserved. | en_US |
dc.relation.ispartof | Journal of Immunological Methods | en_US |
dc.relation.isbasedon | 10.1016/j.jim.2005.07.003 | en_US |
dc.subject.classification | Immunology | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Antigens, CD | en_US |
dc.subject.mesh | Antibodies | en_US |
dc.subject.mesh | Antigens, Surface | en_US |
dc.subject.mesh | Blotting, Western | en_US |
dc.subject.mesh | Immunochemistry | en_US |
dc.subject.mesh | Antibody Specificity | en_US |
dc.title | The HLDA8 blind panel: Findings and conclusions | en_US |
dc.type | Journal Article | |
utslib.citation.volume | 1 | en_US |
utslib.citation.volume | 305 | en_US |
utslib.for | 1107 Immunology | en_US |
utslib.for | 0104 Statistics | en_US |
utslib.for | 1108 Medical Microbiology | en_US |
utslib.for | 1113 Opthalmology and Optometry | en_US |
pubs.embargo.period | Not known | en_US |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
utslib.copyright.status | closed_access | |
pubs.issue | 1 | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 305 | en_US |
Abstract:
There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. © 2005 Elsevier B.V. All rights reserved.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph