The HLDA8 blind panel: Findings and conclusions

Swart, B; Salganik, MP; Wand, MP; Tinckam, K; Milford, EL; Drbal, K; Angelisova, P; Horejsi, V; MacArdle, P; Bailey, S; Hollemweguer, E; Hodge, G; Nairn, J; Millard, D; Dagdeviren, A; Dandie, GW; Zola, H

The HLDA8 blind panel: Findings and conclusions

Swart, B Salganik, MP Wand, MP

Tinckam, K Milford, EL Drbal, K Angelisova, P Horejsi, V MacArdle, P Bailey, S Hollemweguer, E Hodge, G Nairn, J Millard, D Dagdeviren, A Dandie, GW Zola, H

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Publication Type:: Journal Article
Citation:: Journal of Immunological Methods, 2005, 305 (1), pp. 75 - 83
Issue Date:: 2005-10-20

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Field	Value	Language
dc.contributor.author	Swart, B	en_US
dc.contributor.author	Salganik, MP	en_US
dc.contributor.author	Wand, MP https://orcid.org/0000-0003-2555-896X	en_US
dc.contributor.author	Tinckam, K	en_US
dc.contributor.author	Milford, EL	en_US
dc.contributor.author	Drbal, K	en_US
dc.contributor.author	Angelisova, P	en_US
dc.contributor.author	Horejsi, V	en_US
dc.contributor.author	MacArdle, P	en_US
dc.contributor.author	Bailey, S	en_US
dc.contributor.author	Hollemweguer, E	en_US
dc.contributor.author	Hodge, G	en_US
dc.contributor.author	Nairn, J	en_US
dc.contributor.author	Millard, D	en_US
dc.contributor.author	Dagdeviren, A	en_US
dc.contributor.author	Dandie, GW	en_US
dc.contributor.author	Zola, H	en_US
dc.date.available	2005-06-03	en_US
dc.date.issued	2005-10-20	en_US
dc.identifier.citation	Journal of Immunological Methods, 2005, 305 (1), pp. 75 - 83	en_US
dc.identifier.issn	0022-1759	en_US
dc.identifier.uri	http://hdl.handle.net/10453/17008
dc.description.abstract	There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. © 2005 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Journal of Immunological Methods	en_US
dc.relation.isbasedon	10.1016/j.jim.2005.07.003	en_US
dc.subject.classification	Immunology	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Antigens, CD	en_US
dc.subject.mesh	Antibodies	en_US
dc.subject.mesh	Antigens, Surface	en_US
dc.subject.mesh	Blotting, Western	en_US
dc.subject.mesh	Immunochemistry	en_US
dc.subject.mesh	Antibody Specificity	en_US
dc.title	The HLDA8 blind panel: Findings and conclusions	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	305	en_US
utslib.for	1107 Immunology	en_US
utslib.for	0104 Statistics	en_US
utslib.for	1108 Medical Microbiology	en_US
utslib.for	1113 Opthalmology and Optometry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	305	en_US

Abstract:

There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. © 2005 Elsevier B.V. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/17008