Molecular cloning and expression of a chloride ion channel of cell nuclei

Valenzuela, SM; Martin, DK; Por, SB; Robbins, JM; Warton, K; Bootcov, MR; Schofield, PR; Campbell, TJ; Breit, SN

Molecular cloning and expression of a chloride ion channel of cell nuclei

Valenzuela, SM

Martin, DK

Por, SB Robbins, JM Warton, K Bootcov, MR Schofield, PR Campbell, TJ Breit, SN

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Publication Type:: Journal Article
Citation:: Journal of Biological Chemistry, 1997, 272 (19), pp. 12575 - 12582
Issue Date:: 1997-05-09

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Field	Value	Language
dc.contributor.author	Valenzuela, SM https://orcid.org/0000-0001-5934-6047	en_US
dc.contributor.author	Martin, DK https://orcid.org/0000-0001-5913-2372	en_US
dc.contributor.author	Por, SB	en_US
dc.contributor.author	Robbins, JM	en_US
dc.contributor.author	Warton, K	en_US
dc.contributor.author	Bootcov, MR	en_US
dc.contributor.author	Schofield, PR	en_US
dc.contributor.author	Campbell, TJ	en_US
dc.contributor.author	Breit, SN	en_US
dc.date.issued	1997-05-09	en_US
dc.identifier.citation	Journal of Biological Chemistry, 1997, 272 (19), pp. 12575 - 12582	en_US
dc.identifier.issn	0021-9258	en_US
dc.identifier.uri	http://hdl.handle.net/10453/21922
dc.description.abstract	Ion channels are known to be present on the plasma membrane of virtually all cells and have been found on the membranes of various intracellular organelles. However, until recently they were believed not to occur at the nuclear membrane. In this study we describe the molecular cloning and characterization of a nuclear ion channel protein, designated nuclear chloride channel-27 (NCC27), from the human myelomonocytic cell line, U937. NCC27 is a novel chloride ion channel protein that was found to localize principally to the cell nucleus. Its only known homologue is a bovine chloride ion channel protein (p64) believed to localize to internal organelles. NCC27 therefore represents the first human member of a new class of organella chloride ion channel proteins.	en_US
dc.relation.ispartof	Journal of Biological Chemistry	en_US
dc.relation.isbasedon	10.1074/jbc.272.19.12575	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	CHO Cells	en_US
dc.subject.mesh	Cell Nucleus	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cattle	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Chloride Channels	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Blotting, Western	en_US
dc.subject.mesh	Patch-Clamp Techniques	en_US
dc.subject.mesh	Cloning, Molecular	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Open Reading Frames	en_US
dc.subject.mesh	Molecular Weight	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Cricetinae	en_US
dc.title	Molecular cloning and expression of a chloride ion channel of cell nuclei	en_US
dc.type	Journal Article
utslib.citation.volume	19	en_US
utslib.citation.volume	272	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	1004 Medical Biotechnology	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:A1997WY82900047	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	19	en_US
pubs.publication-status	Published	en_US
pubs.volume	272	en_US

Abstract:

Ion channels are known to be present on the plasma membrane of virtually all cells and have been found on the membranes of various intracellular organelles. However, until recently they were believed not to occur at the nuclear membrane. In this study we describe the molecular cloning and characterization of a nuclear ion channel protein, designated nuclear chloride channel-27 (NCC27), from the human myelomonocytic cell line, U937. NCC27 is a novel chloride ion channel protein that was found to localize principally to the cell nucleus. Its only known homologue is a bovine chloride ion channel protein (p64) believed to localize to internal organelles. NCC27 therefore represents the first human member of a new class of organella chloride ion channel proteins.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/21922