The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli

Petty, NK; Bulgin, R; Crepin, VF; Cerdeño-Tárraga, AM; Schroeder, GN; Quail, MA; Lennard, N; Corton, C; Barron, A; Clark, L; Toribio, AL; Parkhill, J; Dougan, G; Frankel, G; Thomson, NR

The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli

Bulgin, R Crepin, VF Cerdeño-Tárraga, AM Schroeder, GN Quail, MA Lennard, N Corton, C Barron, A Clark, L Toribio, AL Parkhill, J Dougan, G Frankel, G Thomson, NR

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2010, 192 (2), pp. 525 - 538
Issue Date:: 2010-01-01

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Field	Value	Language
dc.contributor.author	Petty, NK https://orcid.org/0000-0001-6528-9886	en_US
dc.contributor.author	Bulgin, R	en_US
dc.contributor.author	Crepin, VF	en_US
dc.contributor.author	Cerdeño-Tárraga, AM	en_US
dc.contributor.author	Schroeder, GN	en_US
dc.contributor.author	Quail, MA	en_US
dc.contributor.author	Lennard, N	en_US
dc.contributor.author	Corton, C	en_US
dc.contributor.author	Barron, A	en_US
dc.contributor.author	Clark, L	en_US
dc.contributor.author	Toribio, AL	en_US
dc.contributor.author	Parkhill, J	en_US
dc.contributor.author	Dougan, G	en_US
dc.contributor.author	Frankel, G	en_US
dc.contributor.author	Thomson, NR	en_US
dc.date.issued	2010-01-01	en_US
dc.identifier.citation	Journal of Bacteriology, 2010, 192 (2), pp. 525 - 538	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27805
dc.description.abstract	Citrobacter rodentium (formally Citrobacter freundii biotype 4280) is a highly infectious pathogen that causes colitis and transmissible colonic hyperplasia in mice. In common with enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), C. rodentium exploits a type III secretion system (T3SS) to induce attaching and effacing (A/E) lesions that are essential for virulence. Here, we report the fully annotated genome sequence of the 5.3-Mb chromosome and four plasmids harbored by C. rodentium strain ICC168. The genome sequence revealed key information about the phylogeny of C. rodentium and identified 1,585 C. rodentium-specific (without orthologues in EPEC or EHEC) coding sequences, 10 prophage-like regions, and 17 genomic islands, including the locus for enterocyte effacement (LEE) region, which encodes a T3SS and effector proteins. Among the 29 T3SS effectors found in C. rodentium are all 22 of the core effectors of EPEC strain E2348/69. In addition, we identified a novel C. rodentium effector, named EspS. C. rodentium harbors two type VI secretion systems (T6SS) (CTS1 and CTS2), while EHEC contains only one T6SS (EHS). Our analysis suggests that C. rodentium and EPEC/EHEC have converged on a common host infection strategy through access to a common pool of mobile DNA and that C. rodentium has lost gene functions associated with a previous pathogenic niche. Copyright © 2010, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.01144-09	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Citrobacter rodentium	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Computational Biology	en_US
dc.subject.mesh	Evolution, Molecular	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Male	en_US
dc.title	The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	192	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	192	en_US

Abstract:

Citrobacter rodentium (formally Citrobacter freundii biotype 4280) is a highly infectious pathogen that causes colitis and transmissible colonic hyperplasia in mice. In common with enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), C. rodentium exploits a type III secretion system (T3SS) to induce attaching and effacing (A/E) lesions that are essential for virulence. Here, we report the fully annotated genome sequence of the 5.3-Mb chromosome and four plasmids harbored by C. rodentium strain ICC168. The genome sequence revealed key information about the phylogeny of C. rodentium and identified 1,585 C. rodentium-specific (without orthologues in EPEC or EHEC) coding sequences, 10 prophage-like regions, and 17 genomic islands, including the locus for enterocyte effacement (LEE) region, which encodes a T3SS and effector proteins. Among the 29 T3SS effectors found in C. rodentium are all 22 of the core effectors of EPEC strain E2348/69. In addition, we identified a novel C. rodentium effector, named EspS. C. rodentium harbors two type VI secretion systems (T6SS) (CTS1 and CTS2), while EHEC contains only one T6SS (EHS). Our analysis suggests that C. rodentium and EPEC/EHEC have converged on a common host infection strategy through access to a common pool of mobile DNA and that C. rodentium has lost gene functions associated with a previous pathogenic niche. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/27805