The VQ motif protein IKU1 regulates endosperm growth and seed size in Arabidopsis

Wang, A; Garcia, D; Zhang, H; Feng, K; Chaudhury, A; Berger, F; Peacock, WJ; Dennis, ES; Luo, M

The VQ motif protein IKU1 regulates endosperm growth and seed size in Arabidopsis

Wang, A Garcia, D Zhang, H Feng, K Chaudhury, A Berger, F Peacock, WJ

Dennis, ES

Luo, M

Permalink

Publication Type:: Journal Article
Citation:: Plant Journal, 2010, 63 (4), pp. 670 - 679
Issue Date:: 2010-08-01

Closed Access

	Filename	Description	Size
	2012008161OK.pdf		1 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Wang, A	en_US
dc.contributor.author	Garcia, D	en_US
dc.contributor.author	Zhang, H	en_US
dc.contributor.author	Feng, K	en_US
dc.contributor.author	Chaudhury, A	en_US
dc.contributor.author	Berger, F	en_US
dc.contributor.author	Peacock, WJ https://orcid.org/0000-0001-7314-9074	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.contributor.author	Luo, M	en_US
dc.date.issued	2010-08-01	en_US
dc.identifier.citation	Plant Journal, 2010, 63 (4), pp. 670 - 679	en_US
dc.identifier.issn	0960-7412	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28222
dc.description.abstract	Arabidopsis seed size is regulated by the IKU pathway that includes IKU2 (a leucine-rich repeat kinase) and MINI3 (a WRKY transcription factor). We report the cloning of the IKU1 (At2g35230) gene. iku1 mutants cause reduced endosperm growth and the production of small seeds. IKU1 encodes a protein containing a VQ motif, which is a motif specific to plants. IKU1 is expressed in the early endosperm and its progenitor, the central cell. Restoration of IKU1 function in the endosperm is sufficient to rescue seed size. A genomic construct carrying mutations in the VQ motif failed to complement the iku1 mutation, suggesting an essential role for the VQ motif. IKU1 interacts with MINI3 in the yeast two-hybrid system, consistent with an IKU1 function in the IKU-MINI pathway. Our data support the proposition that endosperm development is an important determinant of seed size. © 2010 Blackwell Publishing Ltd.	en_US
dc.relation.ispartof	Plant Journal	en_US
dc.relation.isbasedon	10.1111/j.1365-313X.2010.04271.x	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Plants, Genetically Modified	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Seeds	en_US
dc.subject.mesh	Protein Kinases	en_US
dc.subject.mesh	Luminescent Proteins	en_US
dc.subject.mesh	Arabidopsis Proteins	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Microscopy, Confocal	en_US
dc.subject.mesh	Two-Hybrid System Techniques	en_US
dc.subject.mesh	Genetic Complementation Test	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Amino Acid Motifs	en_US
dc.subject.mesh	Protein Binding	en_US
dc.subject.mesh	Sequence Homology, Amino Acid	en_US
dc.subject.mesh	Phenotype	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Endosperm	en_US
dc.title	The VQ motif protein IKU1 regulates endosperm growth and seed size in Arabidopsis	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	63	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	63	en_US

Abstract:

Arabidopsis seed size is regulated by the IKU pathway that includes IKU2 (a leucine-rich repeat kinase) and MINI3 (a WRKY transcription factor). We report the cloning of the IKU1 (At2g35230) gene. iku1 mutants cause reduced endosperm growth and the production of small seeds. IKU1 encodes a protein containing a VQ motif, which is a motif specific to plants. IKU1 is expressed in the early endosperm and its progenitor, the central cell. Restoration of IKU1 function in the endosperm is sufficient to rescue seed size. A genomic construct carrying mutations in the VQ motif failed to complement the iku1 mutation, suggesting an essential role for the VQ motif. IKU1 interacts with MINI3 in the yeast two-hybrid system, consistent with an IKU1 function in the IKU-MINI pathway. Our data support the proposition that endosperm development is an important determinant of seed size. © 2010 Blackwell Publishing Ltd.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/28222