Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis

Moldovan, D; Spriggs, A; Yang, J; Pogson, BJ; Dennis, ES; Wilson, IW

Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis

Moldovan, D Spriggs, A Yang, J Pogson, BJ Dennis, ES

Wilson, IW

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Publication Type:: Journal Article
Citation:: Journal of Experimental Botany, 2010, 61 (1), pp. 165 - 177
Issue Date:: 2010-01-01

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Field	Value	Language
dc.contributor.author	Moldovan, D	en_US
dc.contributor.author	Spriggs, A	en_US
dc.contributor.author	Yang, J	en_US
dc.contributor.author	Pogson, BJ	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.contributor.author	Wilson, IW	en_US
dc.date.issued	2010-01-01	en_US
dc.identifier.citation	Journal of Experimental Botany, 2010, 61 (1), pp. 165 - 177	en_US
dc.identifier.issn	0022-0957	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28231
dc.description.abstract	Low-oxygen (hypoxia) stress associated with natural phenomena such as waterlogging, results in widespread transcriptome changes and a metabolic switch from aerobic respiration to anaerobic fermentation. High-throughput sequencing of small RNA libraries obtained from hypoxia-treated and control root tissue identified a total of 65 unique microRNA (miRNA) sequences from 46 families, and 14 trans-acting small interfering RNA (tasiRNA) from three families. Hypoxia resulted in changes to the abundance of 46 miRNAs from 19 families, and all three tasiRNA families. Chemical inhibition of mitochondrial respiration caused similar changes in expression in a majority of the hypoxia-responsive small RNAs analysed. Our data indicate that miRNAs and tasiRNAs play a role in gene regulation and possibly developmental responses to hypoxia, and that a major signal for these responses is likely to be dependent on mitochondrial function.	en_US
dc.relation.ispartof	Journal of Experimental Botany	en_US
dc.relation.isbasedon	10.1093/jxb/erp296	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Mitochondria	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Plant Roots	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	RNA, Small Interfering	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Cell Respiration	en_US
dc.subject.mesh	Cell Hypoxia	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Models, Biological	en_US
dc.subject.mesh	Promoter Regions, Genetic	en_US
dc.subject.mesh	Stress, Physiological	en_US
dc.subject.mesh	High-Throughput Screening Assays	en_US
dc.title	Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	61	en_US
utslib.for	0703 Crop and Pasture Production	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	61	en_US

Abstract:

Low-oxygen (hypoxia) stress associated with natural phenomena such as waterlogging, results in widespread transcriptome changes and a metabolic switch from aerobic respiration to anaerobic fermentation. High-throughput sequencing of small RNA libraries obtained from hypoxia-treated and control root tissue identified a total of 65 unique microRNA (miRNA) sequences from 46 families, and 14 trans-acting small interfering RNA (tasiRNA) from three families. Hypoxia resulted in changes to the abundance of 46 miRNAs from 19 families, and all three tasiRNA families. Chemical inhibition of mitochondrial respiration caused similar changes in expression in a majority of the hypoxia-responsive small RNAs analysed. Our data indicate that miRNAs and tasiRNAs play a role in gene regulation and possibly developmental responses to hypoxia, and that a major signal for these responses is likely to be dependent on mitochondrial function.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/28231