An evolutionarily conserved, alternatively spliced, intron in the p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA

Moore, HC; Johnston, M; Nicol, SM; Bourdon, JC; Thompson, AM; Hutvagner, G; Fuller-Pace, FV

An evolutionarily conserved, alternatively spliced, intron in the p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA

Moore, HC Johnston, M Nicol, SM Bourdon, JC Thompson, AM Hutvagner, G

Fuller-Pace, FV

Permalink

Publication Type:: Journal Article
Citation:: RNA, 2011, 17 (4), pp. 555 - 562
Issue Date:: 2011-04-01

Closed Access

	Filename	Description	Size
	2010003446OK.pdf	Published Version	733.22 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Moore, HC	en_US
dc.contributor.author	Johnston, M	en_US
dc.contributor.author	Nicol, SM	en_US
dc.contributor.author	Bourdon, JC	en_US
dc.contributor.author	Thompson, AM	en_US
dc.contributor.author	Hutvagner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.contributor.author	Fuller-Pace, FV	en_US
dc.date.issued	2011-04-01	en_US
dc.identifier.citation	RNA, 2011, 17 (4), pp. 555 - 562	en_US
dc.identifier.issn	1355-8382	en_US
dc.identifier.uri	http://hdl.handle.net/10453/31165
dc.description.abstract	The DEAD-box RNA helicase p68 (DDX5) plays important roles in several cellular processes, including transcription, pre-mRNA processing, and microRNA (miRNA) processing. p68 expression is growth and developmentally regulated, and alterations in p68 expression and/or function have been implicated in tumor development. The p68 gene encodes an evolutionarily conserved, alternatively spliced, intron the function of which has to date remained unclear. Although the intron-containing p68 RNA does not appear to yield an alternative p68 protein, it is differentially expressed in cell lines and tissues, indicating regulation of expression. Here we show that the p68 conserved intron encodes a novel putative miRNA, suggesting a previously unknown possible regulatory function for the p68 intron. We show that this miRNA (referred to as p68 miRNA) is processed from the intron via the canonical miRNA-processing pathway and that it associates with the Argonaute protein Ago2. Finally we show that the p68 miRNA suppresses an mRNA bearing complementary target sequences, suggesting that it is functional. These findings suggest a novel mechanism by which alterations in p68 expression may impact on the cell. Copyright © 2011 RNA Society.	en_US
dc.relation.ispartof	RNA	en_US
dc.relation.isbasedon	10.1261/rna.2591611	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Dogs	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Evolution, Molecular	en_US
dc.subject.mesh	Alternative Splicing	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Conserved Sequence	en_US
dc.subject.mesh	Introns	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	DEAD-box RNA Helicases	en_US
dc.title	An evolutionarily conserved, alternatively spliced, intron in the p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	17	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
dc.location.activity	WOS:000288545800002
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	17	en_US

Abstract:

The DEAD-box RNA helicase p68 (DDX5) plays important roles in several cellular processes, including transcription, pre-mRNA processing, and microRNA (miRNA) processing. p68 expression is growth and developmentally regulated, and alterations in p68 expression and/or function have been implicated in tumor development. The p68 gene encodes an evolutionarily conserved, alternatively spliced, intron the function of which has to date remained unclear. Although the intron-containing p68 RNA does not appear to yield an alternative p68 protein, it is differentially expressed in cell lines and tissues, indicating regulation of expression. Here we show that the p68 conserved intron encodes a novel putative miRNA, suggesting a previously unknown possible regulatory function for the p68 intron. We show that this miRNA (referred to as p68 miRNA) is processed from the intron via the canonical miRNA-processing pathway and that it associates with the Argonaute protein Ago2. Finally we show that the p68 miRNA suppresses an mRNA bearing complementary target sequences, suggesting that it is functional. These findings suggest a novel mechanism by which alterations in p68 expression may impact on the cell. Copyright © 2011 RNA Society.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/31165