Quantitative expression analysis of selected transcription factors in pavement, basal and trichome cells of mature leaves from Arabidopsis thaliana.

Schliep, M; Ebert, B; Simon-Rosin, U; Zoeller, D; Fisahn, J

Quantitative expression analysis of selected transcription factors in pavement, basal and trichome cells of mature leaves from Arabidopsis thaliana.

Schliep, M Ebert, B Simon-Rosin, U Zoeller, D Fisahn, J

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Publication Type:: Journal Article
Citation:: Protoplasma, 2010, 241 (1-4), pp. 29 - 36
Issue Date:: 2010-05

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Field	Value	Language
dc.contributor.author	Schliep, M	en_US
dc.contributor.author	Ebert, B	en_US
dc.contributor.author	Simon-Rosin, U	en_US
dc.contributor.author	Zoeller, D	en_US
dc.contributor.author	Fisahn, J	en_US
dc.date.available	2009-12-15	en_US
dc.date.issued	2010-05	en_US
dc.identifier.citation	Protoplasma, 2010, 241 (1-4), pp. 29 - 36	en_US
dc.identifier.uri	http://hdl.handle.net/10453/31452
dc.description.abstract	Gene expression levels of several transcription factors from Arabidopsis thaliana that were described previously to be involved in leaf development and trichome formation were analysed in trichome, basal and pavement cells of mature leaves. Single cell samples of these three cells types were collected by glass micro-capillaries. Real-time reverse transcription (RT)-PCR was used to analyse expression patterns of the following transcription factors: MYB23, MYB55, AtHB1, FILAMENTOUS FLOWER (FIL)/YABBY1 (YAB1), TRIPTYCHON (TRY) and CAPRICE (CPC). A difference in the expression patterns of TRY and CPC was revealed. Contrary to the CPC expression pattern, no transcripts of TRY could be detected in pavement cells. FIL/YAB1 was exclusively expressed in trichome cells. AtHB1 was highly expressed throughout all three cell types. MYB55 was higher expressed in basal cells than in trichome and pavement cells. MYB23 showed a pattern of low expression in pavement cells, medium in basal cells and high expression in trichomes. Expression patterns obtained by single cell sampling and real-time RT-PCR were compared to promoter GUS fusions of the selected transcription factors. Therefore, we regenerated two transgenic Arabidopsis lines that expressed the GUS reporter gene under control of the promoters of MYB55 and YAB1. In conclusion, despite their function in leaf morphogenesis, all six transcription factors were detected in mature leaves. Furthermore, single cell sampling and promoter GUS staining patterns demonstrated the predominant presence of MYB55 in basal cells as compared to pavement cells and trichomes.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Protoplasma	en_US
dc.relation.isbasedon	10.1007/s00709-009-0099-7	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Plants, Genetically Modified	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Plant Leaves	en_US
dc.subject.mesh	Proto-Oncogene Proteins c-myb	en_US
dc.subject.mesh	Arabidopsis Proteins	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Reverse Transcriptase Polymerase Chain Reaction	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Models, Biological	en_US
dc.title	Quantitative expression analysis of selected transcription factors in pavement, basal and trichome cells of mature leaves from Arabidopsis thaliana.	en_US
dc.type	Journal Article
utslib.citation.volume	1-4	en_US
utslib.citation.volume	241	en_US
utslib.location.activity	Austria	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	1-4	en_US
pubs.publication-status	Published	en_US
pubs.volume	241	en_US

Abstract:

Gene expression levels of several transcription factors from Arabidopsis thaliana that were described previously to be involved in leaf development and trichome formation were analysed in trichome, basal and pavement cells of mature leaves. Single cell samples of these three cells types were collected by glass micro-capillaries. Real-time reverse transcription (RT)-PCR was used to analyse expression patterns of the following transcription factors: MYB23, MYB55, AtHB1, FILAMENTOUS FLOWER (FIL)/YABBY1 (YAB1), TRIPTYCHON (TRY) and CAPRICE (CPC). A difference in the expression patterns of TRY and CPC was revealed. Contrary to the CPC expression pattern, no transcripts of TRY could be detected in pavement cells. FIL/YAB1 was exclusively expressed in trichome cells. AtHB1 was highly expressed throughout all three cell types. MYB55 was higher expressed in basal cells than in trichome and pavement cells. MYB23 showed a pattern of low expression in pavement cells, medium in basal cells and high expression in trichomes. Expression patterns obtained by single cell sampling and real-time RT-PCR were compared to promoter GUS fusions of the selected transcription factors. Therefore, we regenerated two transgenic Arabidopsis lines that expressed the GUS reporter gene under control of the promoters of MYB55 and YAB1. In conclusion, despite their function in leaf morphogenesis, all six transcription factors were detected in mature leaves. Furthermore, single cell sampling and promoter GUS staining patterns demonstrated the predominant presence of MYB55 in basal cells as compared to pavement cells and trichomes.

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http://hdl.handle.net/10453/31452