Swabs to genomes: A comprehensive workflow

Dunitz, MI; Lang, JM; Jospin, G; Darling, AE; Eisen, JA; Coil, DA

Swabs to genomes: A comprehensive workflow

Dunitz, MI Lang, JM Jospin, G Darling, AE

Eisen, JA Coil, DA

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Publication Type:: Journal Article
Citation:: PeerJ, 2015, 2015 (5)
Issue Date:: 2015-01-01

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Field	Value	Language
dc.contributor.author	Dunitz, MI	en_US
dc.contributor.author	Lang, JM	en_US
dc.contributor.author	Jospin, G	en_US
dc.contributor.author	Darling, AE https://orcid.org/0000-0003-2397-7925	en_US
dc.contributor.author	Eisen, JA	en_US
dc.contributor.author	Coil, DA	en_US
dc.date.available	2015-04-24	en_US
dc.date.issued	2015-01-01	en_US
dc.identifier.citation	PeerJ, 2015, 2015 (5)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/36501
dc.description.abstract	© 2015 Dunitz et al. The sequencing, assembly, and basic analysis of microbial genomes, once a painstaking and expensive undertaking, has become much easier for research labs with access to standard molecular biology and computational tools. However, there are a confusing variety of options available for DNA library preparation and sequencing, and inexperience with bioinformatics can pose a significant barrier to entry for many who may be interested in microbial genomics. The objective of the present study was to design, test, troubleshoot, and publish a simple, comprehensive workflow from the collection of an environmental sample (a swab) to a published microbial genome; empowering even a lab or classroom with limited resources and bioinformatics experience to performit.	en_US
dc.relation.ispartof	PeerJ	en_US
dc.relation.isbasedon	10.7717/peerj.960	en_US
dc.title	Swabs to genomes: A comprehensive workflow	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	2015	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0604 Genetics	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	2015	en_US

Abstract:

© 2015 Dunitz et al. The sequencing, assembly, and basic analysis of microbial genomes, once a painstaking and expensive undertaking, has become much easier for research labs with access to standard molecular biology and computational tools. However, there are a confusing variety of options available for DNA library preparation and sequencing, and inexperience with bioinformatics can pose a significant barrier to entry for many who may be interested in microbial genomics. The objective of the present study was to design, test, troubleshoot, and publish a simple, comprehensive workflow from the collection of an environmental sample (a swab) to a published microbial genome; empowering even a lab or classroom with limited resources and bioinformatics experience to performit.

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http://hdl.handle.net/10453/36501