Chlamydia caviae infection alters abundance but not composition of the guinea pig vaginal microbiota

Neuendorf, E; Gajer, P; Bowlin, AK; Marques, PX; Ma, B; Yang, H; Fu, L; Humphrys, MS; Forney, LJ; Myers, GSA; Bavoil, PM; Rank, RG; Ravel, J

Chlamydia caviae infection alters abundance but not composition of the guinea pig vaginal microbiota

Neuendorf, E Gajer, P Bowlin, AK Marques, PX Ma, B Yang, H Fu, L Humphrys, MS Forney, LJ Myers, GSA

Bavoil, PM Rank, RG Ravel, J

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Publication Type:: Journal Article
Citation:: Pathogens and disease, 2015, 73 (4)
Issue Date:: 2015-06-01

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Field	Value	Language
dc.contributor.author	Neuendorf, E	en_US
dc.contributor.author	Gajer, P	en_US
dc.contributor.author	Bowlin, AK	en_US
dc.contributor.author	Marques, PX	en_US
dc.contributor.author	Ma, B	en_US
dc.contributor.author	Yang, H	en_US
dc.contributor.author	Fu, L	en_US
dc.contributor.author	Humphrys, MS	en_US
dc.contributor.author	Forney, LJ	en_US
dc.contributor.author	Myers, GSA https://orcid.org/0000-0002-4756-4810	en_US
dc.contributor.author	Bavoil, PM	en_US
dc.contributor.author	Rank, RG	en_US
dc.contributor.author	Ravel, J	en_US
dc.date.available	2015-02-26	en_US
dc.date.issued	2015-06-01	en_US
dc.identifier.citation	Pathogens and disease, 2015, 73 (4)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/39723
dc.description.abstract	© FEMS 2015. In humans, the vaginal microbiota is thought to be the first line of defense again pathogens including Chlamydia trachomatis. The guinea pig has been extensively used as a model to study chlamydial infection because it shares anatomical and physiological similarities with humans, such as a squamous vaginal epithelium as well as some of the long-term outcomes caused by chlamydial infection. In this study, we aimed to evaluate the guinea pig-C. caviae model of genital infection as a surrogate for studying the role of the vaginal microbiota in the early steps of C. trachomatis infection in humans. We used culture-independent molecular methods to characterize the relative and absolute abundance of bacterial phylotypes in the guinea pig vaginal microbiota in animals non-infected, mock-infected or infected by C. caviae. We showed that the guinea pig and human vaginal microbiotas are of different bacterial composition and abundance. Chlamydia caviae infection had a profound effect on the absolute abundance of bacterial phylotypes but not on the composition of the guinea pig vaginal microbiota. Our findings compromise the validity of the guinea pig-C. caviae model to study the role of the vaginal microbiota during the early steps of sexually transmitted infection.	en_US
dc.relation.ispartof	Pathogens and disease	en_US
dc.relation.isbasedon	10.1093/femspd/ftv019	en_US
dc.subject.mesh	Vagina	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Guinea Pigs	en_US
dc.subject.mesh	Chlamydia Infections	en_US
dc.subject.mesh	Disease Models, Animal	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Reproductive Tract Infections	en_US
dc.subject.mesh	Microbiota	en_US
dc.title	Chlamydia caviae infection alters abundance but not composition of the guinea pig vaginal microbiota	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	73	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	73	en_US

Abstract:

© FEMS 2015. In humans, the vaginal microbiota is thought to be the first line of defense again pathogens including Chlamydia trachomatis. The guinea pig has been extensively used as a model to study chlamydial infection because it shares anatomical and physiological similarities with humans, such as a squamous vaginal epithelium as well as some of the long-term outcomes caused by chlamydial infection. In this study, we aimed to evaluate the guinea pig-C. caviae model of genital infection as a surrogate for studying the role of the vaginal microbiota in the early steps of C. trachomatis infection in humans. We used culture-independent molecular methods to characterize the relative and absolute abundance of bacterial phylotypes in the guinea pig vaginal microbiota in animals non-infected, mock-infected or infected by C. caviae. We showed that the guinea pig and human vaginal microbiotas are of different bacterial composition and abundance. Chlamydia caviae infection had a profound effect on the absolute abundance of bacterial phylotypes but not on the composition of the guinea pig vaginal microbiota. Our findings compromise the validity of the guinea pig-C. caviae model to study the role of the vaginal microbiota during the early steps of sexually transmitted infection.

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http://hdl.handle.net/10453/39723