Development of a Disposable Label-Free Impedance Immunosensor for Direct and Sensitive Clenbuterol Determination in Pork

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Journal Article
Food Analytical Methods, 2016, 9 (6), pp. 1781 - 1788
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© 2015, Springer Science+Business Media New York. In this work, a portable label-free impedance immunosensor for the analysis of clenbuterol (CLB) was constructed by modifying L-cysteine (L-cys) and glutaraldehyde (GA) on the surface of a screen-printed gold electrode (SPGE). Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize electrochemical properties of the immunosensor. Under the optimized conditions, the immunosensor could detect CLB in the range of 1.0 × 10−4–1.0 mg/L (R = 0.998), and the detection limit is 2.0 × 10−5 mg/L. The as-developed immunosensor can be applied in detecting CLB in real samples. The average recoveries in the spiked pork samples ranged from 92.4 to 104.6 %, and their relative standard deviations were 3.6–5.2 %. The testing results were consistent with those obtained from high-performance liquid chromatography method. The proposed immunosensor exhibits high sensitivity, specificity, and good stability.
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