A study of p53 expression in thermal burns of human skin for determination of wound age
- Publisher:
- Chiltern Publishing
- Publication Type:
- Journal Article
- Citation:
- Tarran Sarah et al. 2004, 'A study of p53 expression in thermal burns of human skin for determination of wound age', Chiltern Publishing, vol. 44, no. 3, pp. 222-226.
- Issue Date:
- 2004
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Early expression of p53 protein in thermal burns of
guinea pig skin has been reported. This study sought
to determine if expression occurred in thermal burns
of human skin and if immunohistochemical demonstration
of p53 protein could be utilised to
distinguish ante-mortem from post-mortem injuries
as well as indicating the age of a lesion in the living
subject.
Biopsy samples were obtained from live patients
and post-mortem examinations. Immunohistochemistry
was uRed to demonstrate the presence of
p53 protein. Staining was assessed by field counting
of epithelial cell nuclei. In live subjects there was a
tendency for early (six hour to five day) expression,
with peak levels occurring around one to two days.
Late samples (13 to 23 days) demonstrated minimal
or no expression. In contrast, burn wounds from
post-mortem examination demonstrated greater
staining for p53 protein in the late (28 to 77 day)
samples than in the early ones. It appears that
expression of p53 protein may assist in the ageing of
ante-mortem, but not post-mortem, bum wounds.
This implies that results obtained from live subjects
may not be applicable to post-mortem material and
that any putative method for determining the age of
a wound should be tested in both situations.
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