Stable Upconversion Nanohybrid Particles for Specific Prostate Cancer Cell Immunodetection

Shi, Y; Shi, B; Dass, AVE; Lu, Y; Sayyadi, N; Kautto, L; Willows, RD; Chung, R; Piper, J; Nevalainen, H; Walsh, B; Jin, D; Packer, NH

Stable Upconversion Nanohybrid Particles for Specific Prostate Cancer Cell Immunodetection

Shi, Y Shi, B Dass, AVE Lu, Y Sayyadi, N

Kautto, L

Willows, RD Chung, R Piper, J Nevalainen, H Walsh, B Jin, D

Packer, NH

Permalink

Publication Type:: Journal Article
Citation:: Scientific Reports, 2016, 6
Issue Date:: 2016-11-22

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published VersionAdobe PDF (1.5 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Shi, Y	en_US
dc.contributor.author	Shi, B	en_US
dc.contributor.author	Dass, AVE	en_US
dc.contributor.author	Lu, Y	en_US
dc.contributor.author	Sayyadi, N https://orcid.org/0000-0002-0044-2170	en_US
dc.contributor.author	Kautto, L https://orcid.org/0000-0002-4724-8818	en_US
dc.contributor.author	Willows, RD	en_US
dc.contributor.author	Chung, R	en_US
dc.contributor.author	Piper, J	en_US
dc.contributor.author	Nevalainen, H	en_US
dc.contributor.author	Walsh, B	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.contributor.author	Packer, NH	en_US
dc.date.available	2016-10-26	en_US
dc.date.issued	2016-11-22	en_US
dc.identifier.citation	Scientific Reports, 2016, 6	en_US
dc.identifier.uri	http://hdl.handle.net/10453/91926
dc.description.abstract	© The Author(s) 2016. Prostate cancer is one of the male killing diseases and early detection of prostate cancer is the key for better treatment and lower cost. However, the number of prostate cancer cells is low at the early stage, so it is very challenging to detect. In this study, we successfully designed and developed upconversion immune-nanohybrids (UINBs) with sustainable stability in a physiological environment, stable optical properties and highly specific targeting capability for early-stage prostate cancer cell detection. The developed UINBs were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS) and luminescence spectroscopy. The targeting function of the biotinylated antibody nanohybrids were confirmed by immunofluorescence assay and western blot analysis. The UINB system is able to specifically detect prostate cancer cells with stable and background-free luminescent signals for highly sensitive prostate cancer cell detection. This work demonstrates a versatile strategy to develop UCNPs based sustainably stable UINBs for sensitive diseased cell detection.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/srep37533	en_US
dc.subject.mesh	Prostate	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Prostatic Neoplasms	en_US
dc.subject.mesh	Microscopy, Electron, Transmission	en_US
dc.subject.mesh	Luminescent Measurements	en_US
dc.subject.mesh	Spectroscopy, Fourier Transform Infrared	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Nanoparticles	en_US
dc.subject.mesh	Early Detection of Cancer	en_US
dc.subject.mesh	Cell Tracking	en_US
dc.title	Stable Upconversion Nanohybrid Particles for Specific Prostate Cancer Cell Immunodetection	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	100709 Nanomedicine	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

© The Author(s) 2016. Prostate cancer is one of the male killing diseases and early detection of prostate cancer is the key for better treatment and lower cost. However, the number of prostate cancer cells is low at the early stage, so it is very challenging to detect. In this study, we successfully designed and developed upconversion immune-nanohybrids (UINBs) with sustainable stability in a physiological environment, stable optical properties and highly specific targeting capability for early-stage prostate cancer cell detection. The developed UINBs were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS) and luminescence spectroscopy. The targeting function of the biotinylated antibody nanohybrids were confirmed by immunofluorescence assay and western blot analysis. The UINB system is able to specifically detect prostate cancer cells with stable and background-free luminescent signals for highly sensitive prostate cancer cell detection. This work demonstrates a versatile strategy to develop UCNPs based sustainably stable UINBs for sensitive diseased cell detection.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/91926