Statistical Design for Optimization and Determination of Tizanidine Hcl using Folin-Ciocalteu (Fc) as Chromogenic Reagent

Dua, K

Statistical Design for Optimization and Determination of Tizanidine Hcl using Folin-Ciocalteu (Fc) as Chromogenic Reagent

Dua, K

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Publisher:: OMICS International
Publication Type:: Journal Article
Citation:: Pharmaceutica Analytica Acta, 2014, 5 (8), pp. 1 - 5
Issue Date:: 2014

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Field	Value	Language
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159	en_US
dc.date.issued	2014	en_US
dc.identifier.citation	Pharmaceutica Analytica Acta, 2014, 5 (8), pp. 1 - 5	en_US
dc.identifier.issn	2153-2435	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116514
dc.description.abstract	A simple, sensitive spectrophotometric method has been developed for quantitative determination of Tizanidine Hydrochloride in bulk and pharmaceutical formulations with application of factorial design. In this method, Tizanidine Hydrochloride is made to react with Folin-Ciocalteu (FC) reagent under alkaline conditions forming a blue chromogen having absorption maximum at 663 nm. Beer’s law was obeyed in the concentration range of 4-36 μg/ml. Results of the analysis were validated as per ICH guidelines and by recovery studies. A 3-factor, 3-level statistical design (Box-Behnken) was used to derive a second-order polynomial equation to construct contour plots for prediction of response. Independent variables studied were the FC-reagent (X1), sodium carbonate (X2) and drug concentration (X3) and the levels of each factor were low, medium, and high. The dependent variable studied was absorbance (Y1). The aims of this study to determination and optimize the Tizanidine HCl using FC as Chromogenic reagent; the design demonstrated the role of the derived equation (polynomial) and two dimensional plots in predicting the values of dependent variable for optimization.	en_US
dc.publisher	OMICS International	en_US
dc.relation.ispartof	Pharmaceutica Analytica Acta	en_US
dc.relation.isbasedon	10.4172/2153-2435.1000307	en_US
dc.title	Statistical Design for Optimization and Determination of Tizanidine Hcl using Folin-Ciocalteu (Fc) as Chromogenic Reagent	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	5	en_US
utslib.for	010401 Applied Statistics	en_US
utslib.for	010405 Statistical Theory	en_US
utslib.for	100403 Medical Molecular Engineering of Nucleic Acids and Proteins	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
utslib.copyright.status	open_access
pubs.consider-herdc	false	en_US
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

A simple, sensitive spectrophotometric method has been developed for quantitative determination of Tizanidine Hydrochloride in bulk and pharmaceutical formulations with application of factorial design. In this method, Tizanidine Hydrochloride is made to react with Folin-Ciocalteu (FC) reagent under alkaline conditions forming a blue chromogen having absorption maximum at 663 nm. Beer’s law was obeyed in the concentration range of 4-36 μg/ml. Results of the analysis were validated as per ICH guidelines and by recovery studies. A 3-factor, 3-level statistical design (Box-Behnken) was used to derive a second-order polynomial equation to construct contour plots for prediction of response. Independent variables studied were the FC-reagent (X1), sodium carbonate (X2) and drug concentration (X3) and the levels of each factor were low, medium, and high. The dependent variable studied was absorbance (Y1). The aims of this study to determination and optimize the Tizanidine HCl using FC as Chromogenic reagent; the design demonstrated the role of the derived equation (polynomial) and two dimensional plots in predicting the values of dependent variable for optimization.

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http://hdl.handle.net/10453/116514