Effect of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction

Li, JY; Zhang, ZC

Effect of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction

Li, JY Zhang, ZC

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Publication Type:: Journal Article
Citation:: Chinese Journal of Tissue Engineering Research, 2013, 17 (32), pp. 5871 - 5876
Issue Date:: 2013-08-06

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Field	Value	Language
dc.contributor.author	Li, JY	en_US
dc.contributor.author	Zhang, ZC	en_US
dc.date.issued	2013-08-06	en_US
dc.identifier.citation	Chinese Journal of Tissue Engineering Research, 2013, 17 (32), pp. 5871 - 5876	en_US
dc.identifier.issn	1673-8225	en_US
dc.identifier.uri	http://hdl.handle.net/10453/116655
dc.description.abstract	Background: Danhong injection, scavenging free radicals and inhibiting lipid peroxidation, can improve microenvironment injury after cerebral infarction. Objective: To explore the influence of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction in rats. Methods: Sixty Wistar rats were selected to prepare models of cerebral infarction by middle cerebral artery occlusion and then randomly divided into control group, bone marrow mesenchymal stem cell group, and combination group. Control group received tail vein injection of PBS. Bone marrow mesenchymal stem cell group received tail vein injection of 2.5×109/L bone marrow mesenchymal stem cell suspension. Combination group received injection of 2.5× 109/L bone marrow mesenchymal stem cell suspension+2 mL/kg Danhong injection, for 5 consecutive days, once a day. Results and Conclusion: There were no significant differences in the neurological dysfunction scores among the three groups at 24 hour and 3 days after implantation (P > 0.05). The neurological dysfunction scores in the ombination group were significantly lower than those in the bone marrow mesenchymal stem cell group and control group at 1 and 2 weeks after transplantation (P < 0.05). In the combination group, GAP-43 and Bcl-2 expression was significantly higher than the bone marrow mesenchymal stem cell group and control group (P < 0.05). Bone marrow mesenchymal stem cell transplantation combined with Danhong injection can significantly promote the local expression of GAP-43 and Bcl-2 after cerebral infarction, and has obvious inhibitory effects on cell apoptosis in rats with cerebral infarction.	en_US
dc.relation.ispartof	Chinese Journal of Tissue Engineering Research	en_US
dc.relation.isbasedon	10.3969/j.issn.2095-4344.2013.32.019	en_US
dc.subject.classification	Neurology & Neurosurgery	en_US
dc.title	Effect of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction	en_US
dc.type	Journal Article
utslib.citation.volume	32	en_US
utslib.citation.volume	17	en_US
utslib.for	0801 Artificial Intelligence and Image Processing	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - AAI - Advanced Analytics Research Centre
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.declined	2017-09-10T15:25:36.505+1000
pubs.issue	32	en_US
pubs.publication-status	Published	en_US
pubs.volume	17	en_US

Abstract:

Background: Danhong injection, scavenging free radicals and inhibiting lipid peroxidation, can improve microenvironment injury after cerebral infarction. Objective: To explore the influence of bone marrow mesenchymal stem cells combined with Danhong injection on expression of GAP-43 and Bcl-2 after cerebral infarction in rats. Methods: Sixty Wistar rats were selected to prepare models of cerebral infarction by middle cerebral artery occlusion and then randomly divided into control group, bone marrow mesenchymal stem cell group, and combination group. Control group received tail vein injection of PBS. Bone marrow mesenchymal stem cell group received tail vein injection of 2.5×109/L bone marrow mesenchymal stem cell suspension. Combination group received injection of 2.5× 109/L bone marrow mesenchymal stem cell suspension+2 mL/kg Danhong injection, for 5 consecutive days, once a day. Results and Conclusion: There were no significant differences in the neurological dysfunction scores among the three groups at 24 hour and 3 days after implantation (P > 0.05). The neurological dysfunction scores in the ombination group were significantly lower than those in the bone marrow mesenchymal stem cell group and control group at 1 and 2 weeks after transplantation (P < 0.05). In the combination group, GAP-43 and Bcl-2 expression was significantly higher than the bone marrow mesenchymal stem cell group and control group (P < 0.05). Bone marrow mesenchymal stem cell transplantation combined with Danhong injection can significantly promote the local expression of GAP-43 and Bcl-2 after cerebral infarction, and has obvious inhibitory effects on cell apoptosis in rats with cerebral infarction.

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http://hdl.handle.net/10453/116655