Box-Behnken design for extraction optimization of crude polysaccharides from Tunisian Phormidium versicolor cyanobacteria (NCC 466): Partial characterization, in vitro antioxidant and antimicrobial activities

Belhaj, D; Frikha, D; Athmouni, K; Jerbi, B; Ahmed, MB; Bouallagui, Z; Kallel, M; Maalej, S; Zhou, J; Ayadi, H

Box-Behnken design for extraction optimization of crude polysaccharides from Tunisian Phormidium versicolor cyanobacteria (NCC 466): Partial characterization, in vitro antioxidant and antimicrobial activities

Belhaj, D Frikha, D Athmouni, K Jerbi, B Ahmed, MB

Bouallagui, Z Kallel, M Maalej, S Zhou, J

Ayadi, H

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Publication Type:: Journal Article
Citation:: International Journal of Biological Macromolecules, 2017, 105 pp. 1501 - 1510
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Belhaj, D	en_US
dc.contributor.author	Frikha, D	en_US
dc.contributor.author	Athmouni, K	en_US
dc.contributor.author	Jerbi, B	en_US
dc.contributor.author	Ahmed, MB https://orcid.org/0000-0003-4756-595X	en_US
dc.contributor.author	Bouallagui, Z	en_US
dc.contributor.author	Kallel, M	en_US
dc.contributor.author	Maalej, S	en_US
dc.contributor.author	Zhou, J https://orcid.org/0000-0003-1393-1608	en_US
dc.contributor.author	Ayadi, H	en_US
dc.date.available	2020-05-25T19:14:52Z
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	International Journal of Biological Macromolecules, 2017, 105 pp. 1501 - 1510	en_US
dc.identifier.issn	0141-8130	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117712
dc.description.abstract	© 2017 In this study, response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize the aqueous extraction of crude polysaccharides from Tunisian cyanobacteria Phormidium versicolor (NCC 466). The optimal extraction conditions with an extraction yield of 21.56 ± 0.92% were as follows: extraction temperature at 81.05 °C, extraction time of 3.99 h, and water to raw material ratio of 21.52 mL g−1. Crude Phormidium versicolor polysaccharides (CPv-PS) are found to be a hetero-sulfated-anionic polysaccharides that contained carbohydrate (79.37 ± 1.58%), protein (0.45 ± 0.11%), uronic acids (4.37 ± 0.19%) and sulfate (6.83 ± 0.28%). The carbohydrate fraction was composed of arabinose, xylose, ribose, rhamnose, N-acetyl glucosamine, galactose, glucose, mannose, glucuronic acid and saccharose with corresponding mole percentages of 2.41, 14.58, 2.18, 6.23, 7.04, 28.21, 26.04, 3.02, 0.86 and 5.07, respectively. Evaluation of the antioxidant activity in vitro suggested that CPv-PS strongly scavenged radicals, prevented bleaching of β-carotene and reduced activity. Furthermore, the CPv-PS exhibited effective antimicrobial properties.	en_US
dc.relation.ispartof	International Journal of Biological Macromolecules	en_US
dc.relation.isbasedon	10.1016/j.ijbiomac.2017.06.046	en_US
dc.subject.classification	Polymers	en_US
dc.subject.mesh	Cyanobacteria	en_US
dc.subject.mesh	Hydroxyl Radical	en_US
dc.subject.mesh	Iron	en_US
dc.subject.mesh	Water	en_US
dc.subject.mesh	Biphenyl Compounds	en_US
dc.subject.mesh	beta Carotene	en_US
dc.subject.mesh	Picrates	en_US
dc.subject.mesh	Polysaccharides, Bacterial	en_US
dc.subject.mesh	Antioxidants	en_US
dc.subject.mesh	Anti-Infective Agents	en_US
dc.subject.mesh	Chemical Fractionation	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Oxidation-Reduction	en_US
dc.title	Box-Behnken design for extraction optimization of crude polysaccharides from Tunisian Phormidium versicolor cyanobacteria (NCC 466): Partial characterization, in vitro antioxidant and antimicrobial activities	en_US
dc.type	Journal Article
utslib.citation.volume	105	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	105	en_US

Abstract:

© 2017 In this study, response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize the aqueous extraction of crude polysaccharides from Tunisian cyanobacteria Phormidium versicolor (NCC 466). The optimal extraction conditions with an extraction yield of 21.56 ± 0.92% were as follows: extraction temperature at 81.05 °C, extraction time of 3.99 h, and water to raw material ratio of 21.52 mL g−1. Crude Phormidium versicolor polysaccharides (CPv-PS) are found to be a hetero-sulfated-anionic polysaccharides that contained carbohydrate (79.37 ± 1.58%), protein (0.45 ± 0.11%), uronic acids (4.37 ± 0.19%) and sulfate (6.83 ± 0.28%). The carbohydrate fraction was composed of arabinose, xylose, ribose, rhamnose, N-acetyl glucosamine, galactose, glucose, mannose, glucuronic acid and saccharose with corresponding mole percentages of 2.41, 14.58, 2.18, 6.23, 7.04, 28.21, 26.04, 3.02, 0.86 and 5.07, respectively. Evaluation of the antioxidant activity in vitro suggested that CPv-PS strongly scavenged radicals, prevented bleaching of β-carotene and reduced activity. Furthermore, the CPv-PS exhibited effective antimicrobial properties.

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http://hdl.handle.net/10453/117712