Characterizing dynamic properties of retinal vessels in the rat eye using high speed imaging

Golzan, SM; Butlin, M; Kouchaki, Z; Gupta, V; Avolio, A; Graham, SL

Characterizing dynamic properties of retinal vessels in the rat eye using high speed imaging

Golzan, SM

Butlin, M Kouchaki, Z Gupta, V Avolio, A Graham, SL

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Publication Type:: Journal Article
Citation:: Microvascular Research, 2014, 92 pp. 56 - 61
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Golzan, SM https://orcid.org/0000-0002-4479-3917	en_US
dc.contributor.author	Butlin, M	en_US
dc.contributor.author	Kouchaki, Z	en_US
dc.contributor.author	Gupta, V	en_US
dc.contributor.author	Avolio, A	en_US
dc.contributor.author	Graham, SL	en_US
dc.date.available	2013-12-23	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Microvascular Research, 2014, 92 pp. 56 - 61	en_US
dc.identifier.issn	0026-2862	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118152
dc.description.abstract	Purpose: The dynamic properties of retinal vessels including pulse wave propagation and pulsatility index provide new perspective in retinal hemodynamic analysis. In this study we utilize a high speed imaging system to capture these characteristics in the rat eye for the first time. Methods: Retinal video images of 9 Wistar-Kyoto (WKY) rats were captured at a rate of 250 frames per second for 10. s with a 50° field of view using a high speed camera (Optronis, Kehl, Germany). Two recordings were taken from each rat at the same sites for repeatability analysis. The electrocardiogram (ECG) was measured simultaneously with retinal images. Arterial retinal pulse wave velocity (rPWV) and arterial/venous pulse amplitude were calculated from recorded images. Arterial measurements were repeated in another normotensive strain of the same age (Sprague-Dawley, n = 4). Results: The average WKY rPWV was 11.4±6.1cm/s. The differences between repeated measures were not significant (-2.8±2.9cm/s, p=0.2). Sprague-Dawley animals had a similar rPWV (9.8±2.2cm/s, p=0.61). The average arterial and venous pulse amplitude was 7.1±1.5μm and 8.2±2.0μm respectively. There was a positive correlation between rPWV and heart rate in the WKY groups (r2=0.32). A positive correlation was also obtained between arterial and venous diameter and their pulse amplitude (r2=0.67 and r2=0.37 respectively). Conclusion: rPWV was associated with heart rate. Higher pulsation amplitude was also correlated with larger vessel diameter. High speed imaging of retinal vessels in the rat eye provides an accurate and robust method to study dynamic characteristics of these vessels and their relationship with ocular and systemic abnormalities. © 2013 Elsevier Inc.	en_US
dc.relation.ispartof	Microvascular Research	en_US
dc.relation.isbasedon	10.1016/j.mvr.2013.12.006	en_US
dc.subject.classification	Cardiovascular System & Hematology	en_US
dc.subject.mesh	Retinal Vessels	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats, Inbred WKY	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Rats, Sprague-Dawley	en_US
dc.subject.mesh	Diagnostic Techniques, Ophthalmological	en_US
dc.subject.mesh	Models, Animal	en_US
dc.subject.mesh	Video Recording	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Hemodynamics	en_US
dc.subject.mesh	Pulse Wave Analysis	en_US
dc.title	Characterizing dynamic properties of retinal vessels in the rat eye using high speed imaging	en_US
dc.type	Journal Article
utslib.citation.volume	92	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	92	en_US

Abstract:

Purpose: The dynamic properties of retinal vessels including pulse wave propagation and pulsatility index provide new perspective in retinal hemodynamic analysis. In this study we utilize a high speed imaging system to capture these characteristics in the rat eye for the first time. Methods: Retinal video images of 9 Wistar-Kyoto (WKY) rats were captured at a rate of 250 frames per second for 10. s with a 50° field of view using a high speed camera (Optronis, Kehl, Germany). Two recordings were taken from each rat at the same sites for repeatability analysis. The electrocardiogram (ECG) was measured simultaneously with retinal images. Arterial retinal pulse wave velocity (rPWV) and arterial/venous pulse amplitude were calculated from recorded images. Arterial measurements were repeated in another normotensive strain of the same age (Sprague-Dawley, n = 4). Results: The average WKY rPWV was 11.4±6.1cm/s. The differences between repeated measures were not significant (-2.8±2.9cm/s, p=0.2). Sprague-Dawley animals had a similar rPWV (9.8±2.2cm/s, p=0.61). The average arterial and venous pulse amplitude was 7.1±1.5μm and 8.2±2.0μm respectively. There was a positive correlation between rPWV and heart rate in the WKY groups (r2=0.32). A positive correlation was also obtained between arterial and venous diameter and their pulse amplitude (r2=0.67 and r2=0.37 respectively). Conclusion: rPWV was associated with heart rate. Higher pulsation amplitude was also correlated with larger vessel diameter. High speed imaging of retinal vessels in the rat eye provides an accurate and robust method to study dynamic characteristics of these vessels and their relationship with ocular and systemic abnormalities. © 2013 Elsevier Inc.

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