Absorption and fluorescence spectroscopy on a smartphone
- Publication Type:
- Conference Proceeding
- Proceedings of SPIE - The International Society for Optical Engineering, 2015, 9655
- Issue Date:
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© 2015 Copyright SPIE. A self-powered smartphone-based field-portable "dual" spectrometer has been developed for both absorption and fluorescence measurements. The smartphone's existing flash LED has sufficient optical irradiance to undertake absorption measurements within a 3D-printed case containing a low cost nano-imprinted polymer diffraction grating. A UV (λ
ex ∼ 370 nm) and VIS (λ ex ∼ 450 nm) LED are wired into the circuit of the flash LED to provide an excitation source for fluorescence measurements. Using a customized app on the smartphone, measurements of absorption and fluorescence spectra are demonstrated using pH-sensitive and Zn2+-responsive probes. Detection over a 300 nm span with 0.42 nm/pixel spectral resolution is demonstrated. Despite the low cost and small size of the portable spectrometer, the results compare well with bench top instruments.
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