Functional photoacoustic micro-imaging of cerebral hemodynamic changes in single blood vessels after photo-induced brain stroke

Liao, LD; Chen, YY; Lin, CT; Li, ML

Functional photoacoustic micro-imaging of cerebral hemodynamic changes in single blood vessels after photo-induced brain stroke

Liao, LD Chen, YY Lin, CT

Li, ML

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Publication Type:: Conference Proceeding
Citation:: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2013, 8581
Issue Date:: 2013-05-28

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Field	Value	Language
dc.contributor.author	Liao, LD	en_US
dc.contributor.author	Chen, YY	en_US
dc.contributor.author	Lin, CT https://orcid.org/0000-0001-8371-8197	en_US
dc.contributor.author	Li, ML	en_US
dc.date.issued	2013-05-28	en_US
dc.identifier.citation	Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2013, 8581	en_US
dc.identifier.isbn	9780819493507	en_US
dc.identifier.issn	1605-7422	en_US
dc.identifier.uri	http://hdl.handle.net/10453/120200
dc.description.abstract	Studying the functional hemodynamic roles of individual cerebral cortical arterioles in maintaining both the structure and function of cortical regions during and after brain stroke in small animals is an important issue. Recently, functional photoacoustic microscopy (fPAM) has been proved as a reliable imaging technique to probe the total hemoglobin concentration (HbT), cerebral blood volume (CBV) and hemoglobin oxygen saturation (SO2) in single cerebral blood vessels of rats. Here, we report the application of fPAM associated with electrophysiology recordings to investigating functional hemodynamic changes in single cortical arterioles of rats with electrical forepaw stimulation after photo-induced ischemic stroke. Because of the weak optical focusing nature of our fPAM system, photo-induced ischemic stroke targeting single cortical arterioles can be easily conducted with simple adaptation. Functional HbT, CBV and SO2 changes associated with the induced stroke in selected arterioles from the anterior cerebral artery system were imaged with 36 x 65-μm spatial resolution. Experimental results showed that after photo-occlusion of a single arteriole, the functional changes of nearby arterioles in cerebral cortex only can be observed immediately after the stroke. After a few minutes of stroke onset, there are no significant functional changes under the forepaw stimulation, suggesting that alternate blood flow routes are not actively recruited. The fPAM with electrophysiology recordings complements existing imaging techniques and has the potential to offer a favorable tool for explicitly studying cerebral hemodynamics in small animal models of photo-indcued ischemic stroke. © 2013 Copyright SPIE.	en_US
dc.relation.ispartof	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en_US
dc.relation.isbasedon	10.1117/12.2003543	en_US
dc.title	Functional photoacoustic micro-imaging of cerebral hemodynamic changes in single blood vessels after photo-induced brain stroke	en_US
dc.type	Conference Proceeding
utslib.citation.volume	8581	en_US
utslib.for	0906 Electrical and Electronic Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Computer Science
pubs.organisational-group	/University of Technology Sydney/Strength - CAI - Centre for Artificial Intelligence
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	8581	en_US

Abstract:

Studying the functional hemodynamic roles of individual cerebral cortical arterioles in maintaining both the structure and function of cortical regions during and after brain stroke in small animals is an important issue. Recently, functional photoacoustic microscopy (fPAM) has been proved as a reliable imaging technique to probe the total hemoglobin concentration (HbT), cerebral blood volume (CBV) and hemoglobin oxygen saturation (SO2) in single cerebral blood vessels of rats. Here, we report the application of fPAM associated with electrophysiology recordings to investigating functional hemodynamic changes in single cortical arterioles of rats with electrical forepaw stimulation after photo-induced ischemic stroke. Because of the weak optical focusing nature of our fPAM system, photo-induced ischemic stroke targeting single cortical arterioles can be easily conducted with simple adaptation. Functional HbT, CBV and SO2 changes associated with the induced stroke in selected arterioles from the anterior cerebral artery system were imaged with 36 x 65-μm spatial resolution. Experimental results showed that after photo-occlusion of a single arteriole, the functional changes of nearby arterioles in cerebral cortex only can be observed immediately after the stroke. After a few minutes of stroke onset, there are no significant functional changes under the forepaw stimulation, suggesting that alternate blood flow routes are not actively recruited. The fPAM with electrophysiology recordings complements existing imaging techniques and has the potential to offer a favorable tool for explicitly studying cerebral hemodynamics in small animal models of photo-indcued ischemic stroke. © 2013 Copyright SPIE.

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http://hdl.handle.net/10453/120200