The role of microbial diversity and composition in minimizing sludge production in the oxic-settling-anoxic process

Semblante, GU; Phan, HV; Hai, FI; Xu, ZQ; Price, WE; Nghiem, LD

The role of microbial diversity and composition in minimizing sludge production in the oxic-settling-anoxic process

Semblante, GU Phan, HV Hai, FI Xu, ZQ Price, WE Nghiem, LD

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Publication Type:: Journal Article
Citation:: Science of the Total Environment, 2017, 607-608 pp. 558 - 567
Issue Date:: 2017-12-31

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Field	Value	Language
dc.contributor.author	Semblante, GU	en_US
dc.contributor.author	Phan, HV	en_US
dc.contributor.author	Hai, FI	en_US
dc.contributor.author	Xu, ZQ	en_US
dc.contributor.author	Price, WE	en_US
dc.contributor.author	Nghiem, LD https://orcid.org/0000-0002-9039-5792	en_US
dc.date.available	2020-05-25T19:13:57Z
dc.date.issued	2017-12-31	en_US
dc.identifier.citation	Science of the Total Environment, 2017, 607-608 pp. 558 - 567	en_US
dc.identifier.issn	0048-9697	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123942
dc.description.abstract	© 2017 Elsevier B.V. The oxic-settling-anoxic (OSA) process, which involves an aerobic tank attached to oxygen- and substrate-deficient external anoxic reactors, minimizes sludge production in biological wastewater treatment. In this study, the microbial community structure of OSA was determined. Principal coordinate analysis showed that among the three operational factors, i.e., (i) redox condition, (ii) external reactor sludge retention time (SRText), and (iii) sludge interchange between aerobic and anoxic reactors, redox condition had the greatest impact on microbial diversity. Generally, reactors with lower oxidation-reduction potential had higher microbial diversity. The main aerobic sequencing batch reactor of OSA (SBROSA) that interchanged sludge with an external anoxic reactor had greater microbial diversity than SBRcontrol which did not have sludge interchange. SBROSA sustained high abundance of the slow-growing nitrifying bacteria (e.g., Nitrospirales and Nitrosomondales) and consequently exhibited reduced sludge yield. Specific groups of bacteria facilitated sludge autolysis in the external reactors. Hydrolyzing (e.g., Bacteroidetes and Chloroflexi) and fermentative (e.g., Firmicutes) bacteria, which can break down cellular matter, proliferated in both the external aerobic/anoxic and anoxic reactors. Sludge autolysis in the anoxic reactor was enhanced with the increase of predatory bacteria (e.g., order Myxobacteriales and genus Bdellovibrio) that can contribute to biomass decay. Furthermore, β- and γ-Proteobacteria were identified as the bacterial phyla that primarily underwent decay in the external reactors.	en_US
dc.relation.ispartof	Science of the Total Environment	en_US
dc.relation.isbasedon	10.1016/j.scitotenv.2017.06.253	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.title	The role of microbial diversity and composition in minimizing sludge production in the oxic-settling-anoxic process	en_US
dc.type	Journal Article
utslib.citation.volume	607-608	en_US
utslib.for	0203 Classical Physics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	607-608	en_US

Abstract:

© 2017 Elsevier B.V. The oxic-settling-anoxic (OSA) process, which involves an aerobic tank attached to oxygen- and substrate-deficient external anoxic reactors, minimizes sludge production in biological wastewater treatment. In this study, the microbial community structure of OSA was determined. Principal coordinate analysis showed that among the three operational factors, i.e., (i) redox condition, (ii) external reactor sludge retention time (SRText), and (iii) sludge interchange between aerobic and anoxic reactors, redox condition had the greatest impact on microbial diversity. Generally, reactors with lower oxidation-reduction potential had higher microbial diversity. The main aerobic sequencing batch reactor of OSA (SBROSA) that interchanged sludge with an external anoxic reactor had greater microbial diversity than SBRcontrol which did not have sludge interchange. SBROSA sustained high abundance of the slow-growing nitrifying bacteria (e.g., Nitrospirales and Nitrosomondales) and consequently exhibited reduced sludge yield. Specific groups of bacteria facilitated sludge autolysis in the external reactors. Hydrolyzing (e.g., Bacteroidetes and Chloroflexi) and fermentative (e.g., Firmicutes) bacteria, which can break down cellular matter, proliferated in both the external aerobic/anoxic and anoxic reactors. Sludge autolysis in the anoxic reactor was enhanced with the increase of predatory bacteria (e.g., order Myxobacteriales and genus Bdellovibrio) that can contribute to biomass decay. Furthermore, β- and γ-Proteobacteria were identified as the bacterial phyla that primarily underwent decay in the external reactors.

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http://hdl.handle.net/10453/123942