Evaluation of in vitro and in vivo anti-urolithiatic activity of silver nanoparticles containing aqueous leaf extract of Tragia involucrata

Velu, V; Das, M; Raj N, AN; Dua, K; Malipeddi, H

Evaluation of in vitro and in vivo anti-urolithiatic activity of silver nanoparticles containing aqueous leaf extract of Tragia involucrata

Velu, V Das, M Raj N, AN Dua, K

Malipeddi, H

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Publication Type:: Journal Article
Citation:: Drug Delivery and Translational Research, 2017, 7 (3), pp. 439 - 449
Issue Date:: 2017-06-01

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Field	Value	Language
dc.contributor.author	Velu, V	en_US
dc.contributor.author	Das, M	en_US
dc.contributor.author	Raj N, AN	en_US
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159	en_US
dc.contributor.author	Malipeddi, H	en_US
dc.date.issued	2017-06-01	en_US
dc.identifier.citation	Drug Delivery and Translational Research, 2017, 7 (3), pp. 439 - 449	en_US
dc.identifier.issn	2190-393X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124658
dc.description.abstract	© 2017, Controlled Release Society. The present investigation is focused on exploring the anti-urolithiatic potential of aqueous leaf extract of Tragia involucrata (TIA) and its silver nanoparticles (AgNPs) and to quantify the total phenol, flavonoid, terpenoid and sterol contents present in TIA. Quantification results suggested TIA to be a rich source of phenol, flavonoid and terpenoid and less of sterol content. The AgNPs were synthesized by a simple green method using aqueous extract of T. involucrata. The formation of AgNPs was confirmed through UV spectroscopy, particle size analysis, zeta potential, X-ray diffraction and transmission electron microscopy. The in vitro struvite growth inhibitory activity of the extract was performed using a single gel diffusion method. Samples incorporated with higher concentration of 2% TIA and AgNPs (200 μg mL−1) exhibited potent crystal growth inhibitory activity which was further supported by the dissolution of crystals in gel medium. Calcium oxalate stone formation was induced in rats by the oral administration of ethylene glycol in water. Stone formation was assessed by increase in the levels of calcium and phosphorous in the urine and accumulation of nitrogenous substances like urea, creatinine in renal tissues and blood. Prophylactic treatment with TIA and AgNPs showed significant anti-urolithiatic activity with normalization of the mineral contents of the urine and serum samples. Histopathological analysis of the kidney of TIA- and AgNP-treated animals showed no CaOx deposits and a normal architecture of the kidney cells. We conclude that aqueous extract of T. involucrata and its AgNPs has potential for the treatment of patients with recurrent stones.	en_US
dc.relation.ispartof	Drug Delivery and Translational Research	en_US
dc.relation.isbasedon	10.1007/s13346-017-0363-x	en_US
dc.subject.mesh	Kidney	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats, Wistar	en_US
dc.subject.mesh	Euphorbiaceae	en_US
dc.subject.mesh	Plant Leaves	en_US
dc.subject.mesh	Silver	en_US
dc.subject.mesh	Calcium Oxalate	en_US
dc.subject.mesh	Gout Suppressants	en_US
dc.subject.mesh	Plant Extracts	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Urolithiasis	en_US
dc.subject.mesh	Metal Nanoparticles	en_US
dc.subject.mesh	Struvite	en_US
dc.title	Evaluation of in vitro and in vivo anti-urolithiatic activity of silver nanoparticles containing aqueous leaf extract of Tragia involucrata	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	7	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

© 2017, Controlled Release Society. The present investigation is focused on exploring the anti-urolithiatic potential of aqueous leaf extract of Tragia involucrata (TIA) and its silver nanoparticles (AgNPs) and to quantify the total phenol, flavonoid, terpenoid and sterol contents present in TIA. Quantification results suggested TIA to be a rich source of phenol, flavonoid and terpenoid and less of sterol content. The AgNPs were synthesized by a simple green method using aqueous extract of T. involucrata. The formation of AgNPs was confirmed through UV spectroscopy, particle size analysis, zeta potential, X-ray diffraction and transmission electron microscopy. The in vitro struvite growth inhibitory activity of the extract was performed using a single gel diffusion method. Samples incorporated with higher concentration of 2% TIA and AgNPs (200 μg mL−1) exhibited potent crystal growth inhibitory activity which was further supported by the dissolution of crystals in gel medium. Calcium oxalate stone formation was induced in rats by the oral administration of ethylene glycol in water. Stone formation was assessed by increase in the levels of calcium and phosphorous in the urine and accumulation of nitrogenous substances like urea, creatinine in renal tissues and blood. Prophylactic treatment with TIA and AgNPs showed significant anti-urolithiatic activity with normalization of the mineral contents of the urine and serum samples. Histopathological analysis of the kidney of TIA- and AgNP-treated animals showed no CaOx deposits and a normal architecture of the kidney cells. We conclude that aqueous extract of T. involucrata and its AgNPs has potential for the treatment of patients with recurrent stones.

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http://hdl.handle.net/10453/124658