Unraveling microbial structure and diversity of activated sludge in a full-scale simultaneous nitrogen and phosphorus removal plant using metagenomic sequencing

Guo, J; Ni, BJ; Han, X; Chen, X; Bond, P; Peng, Y; Yuan, Z

Unraveling microbial structure and diversity of activated sludge in a full-scale simultaneous nitrogen and phosphorus removal plant using metagenomic sequencing

Guo, J Ni, BJ

Han, X Chen, X Bond, P Peng, Y Yuan, Z

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Publication Type:: Journal Article
Citation:: Enzyme and Microbial Technology, 2017, 102 pp. 16 - 25
Issue Date:: 2017-07-01

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Field	Value	Language
dc.contributor.author	Guo, J	en_US
dc.contributor.author	Ni, BJ https://orcid.org/0000-0002-1129-7837	en_US
dc.contributor.author	Han, X	en_US
dc.contributor.author	Chen, X	en_US
dc.contributor.author	Bond, P	en_US
dc.contributor.author	Peng, Y	en_US
dc.contributor.author	Yuan, Z	en_US
dc.date.available	2017-03-20	en_US
dc.date.issued	2017-07-01	en_US
dc.identifier.citation	Enzyme and Microbial Technology, 2017, 102 pp. 16 - 25	en_US
dc.identifier.issn	0141-0229	en_US
dc.identifier.uri	http://hdl.handle.net/10453/126671
dc.description.abstract	© 2017 Activated sludge contains highly complex microbial communities, which play crucial roles in pollutant removal performance in wastewater treatment plants (WWTPs). Metagenomic sequencing was applied to characterize microbial community and functional profiles within activated sludge from a full-scale municipal WWTP carrying out simultaneous nitrogen and phosphorous removal (SNPR). We applied the assembled contigs (N90 of 591 bp) and predicted genes to conduct taxonomic and function annotations, respectively. Results revealed the extraordinary microbial diversity of activated sludge, which included detection of minority populations that are difficult to be explored by traditional molecular methods. Taxonomic analysis indicated that the dominant bacterial phyla were Proteobacteria, Nitrospirae, Bacteroidetes, Actinobacteria and Firmicutes. The abundance of the key organisms involved in nitrogen and phosphorous removal were qualified. Aerobic ammonia-oxidizing bacteria distinctly dominate over ammonia-oxidizing archaea and anaerobic ammonium oxidation bacteria. Various key enzymes involved in the global nitrogen cycle were annotated in the activated sludge. High abundance of the known polyphosphate accumulating organisms was detected (approximately 4.89% of the overall population reads), supporting good phosphorous removal performance. This study provides a comprehensive insight into the community structure and diversity of the SNPR system, and will provide foundation for optimal operation of nutrient removal systems.	en_US
dc.relation.ispartof	Enzyme and Microbial Technology	en_US
dc.relation.isbasedon	10.1016/j.enzmictec.2017.03.009	en_US
dc.subject.classification	Biotechnology	en_US
dc.subject.mesh	Nitrogen	en_US
dc.subject.mesh	Phosphorus	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Industrial Microbiology	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Sewage	en_US
dc.subject.mesh	Waste Disposal, Fluid	en_US
dc.subject.mesh	Biodegradation, Environmental	en_US
dc.subject.mesh	Metagenomics	en_US
dc.subject.mesh	Microbial Consortia	en_US
dc.subject.mesh	Waste Water	en_US
dc.title	Unraveling microbial structure and diversity of activated sludge in a full-scale simultaneous nitrogen and phosphorus removal plant using metagenomic sequencing	en_US
dc.type	Journal Article
utslib.citation.volume	102	en_US
utslib.for	1002 Environmental Biotechnology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	102	en_US

Abstract:

© 2017 Activated sludge contains highly complex microbial communities, which play crucial roles in pollutant removal performance in wastewater treatment plants (WWTPs). Metagenomic sequencing was applied to characterize microbial community and functional profiles within activated sludge from a full-scale municipal WWTP carrying out simultaneous nitrogen and phosphorous removal (SNPR). We applied the assembled contigs (N90 of 591 bp) and predicted genes to conduct taxonomic and function annotations, respectively. Results revealed the extraordinary microbial diversity of activated sludge, which included detection of minority populations that are difficult to be explored by traditional molecular methods. Taxonomic analysis indicated that the dominant bacterial phyla were Proteobacteria, Nitrospirae, Bacteroidetes, Actinobacteria and Firmicutes. The abundance of the key organisms involved in nitrogen and phosphorous removal were qualified. Aerobic ammonia-oxidizing bacteria distinctly dominate over ammonia-oxidizing archaea and anaerobic ammonium oxidation bacteria. Various key enzymes involved in the global nitrogen cycle were annotated in the activated sludge. High abundance of the known polyphosphate accumulating organisms was detected (approximately 4.89% of the overall population reads), supporting good phosphorous removal performance. This study provides a comprehensive insight into the community structure and diversity of the SNPR system, and will provide foundation for optimal operation of nutrient removal systems.

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http://hdl.handle.net/10453/126671