Dark fermentative hydrogen production following the sequential dilute acid pretreatment and enzymatic saccharification of rice husk

Publication Type:
Journal Article
Citation:
International Journal of Hydrogen Energy, 2017, 42 (45), pp. 27577 - 27583
Issue Date:
2017-01-01
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© 2017 Hydrogen Energy Publications LLC This study examined the effect of sequential dilute acid hydrolysis and enzyme saccharification conditions of rice husk on hydrogen (H2) fermentation. The rice husk was hydrolyzed using dilute H2SO4and then further enzymatically hydrolyzed with various concentrations (0.1, 0.25, 0.5, 0.75, and 1.0 mg protein mL−1) of Celluclast 1.5 L®. The sequential pretreatment significantly enhanced sugar recovery, up to 174% for 1.0 mg mL−1concentration of the enzyme. Increasing the concentration of enzyme slightly increases sugar recovery. While the production of furfural and 5-hydroxymethylfurfural occurred during dilute acid hydrolysis, it was not observed during enzymatic saccharification. At the following H2fermentation of the hydrolyzates, H2yield was improved up to 150% by using the dilute acid and enzymatic hydrolyzate as substrate, as compared when only dilute acid hydrolyzate was used. The highest H2yield of 473.1 mL H2g−1rice husk was achieved with enzyme concentration of 0.75 mg protein mL−1.
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