Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

Peng, H; Tang, J; Xiao, H; Bria, A; Zhou, J; Butler, V; Zhou, Z; Gonzalez-Bellido, PT; Oh, SW; Chen, J; Mitra, A; Tsien, RW; Zeng, H; Ascoli, GA; Iannello, G; Hawrylycz, M; Myers, E; Long, F

Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

Peng, H Tang, J Xiao, H Bria, A Zhou, J

Butler, V Zhou, Z Gonzalez-Bellido, PT Oh, SW Chen, J Mitra, A Tsien, RW Zeng, H Ascoli, GA Iannello, G Hawrylycz, M Myers, E Long, F

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Publication Type:: Journal Article
Citation:: Nature Communications, 2014, 5
Issue Date:: 2014-07-11

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Field	Value	Language
dc.contributor.author	Peng, H	en_US
dc.contributor.author	Tang, J	en_US
dc.contributor.author	Xiao, H	en_US
dc.contributor.author	Bria, A	en_US
dc.contributor.author	Zhou, J https://orcid.org/0000-0001-6034-644X	en_US
dc.contributor.author	Butler, V	en_US
dc.contributor.author	Zhou, Z	en_US
dc.contributor.author	Gonzalez-Bellido, PT	en_US
dc.contributor.author	Oh, SW	en_US
dc.contributor.author	Chen, J	en_US
dc.contributor.author	Mitra, A	en_US
dc.contributor.author	Tsien, RW	en_US
dc.contributor.author	Zeng, H	en_US
dc.contributor.author	Ascoli, GA	en_US
dc.contributor.author	Iannello, G	en_US
dc.contributor.author	Hawrylycz, M	en_US
dc.contributor.author	Myers, E	en_US
dc.contributor.author	Long, F	en_US
dc.date.available	2014-06-09	en_US
dc.date.issued	2014-07-11	en_US
dc.identifier.citation	Nature Communications, 2014, 5	en_US
dc.identifier.uri	http://hdl.handle.net/10453/131815
dc.description.abstract	Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain. © 2014 Macmillan Publishers Limited. All rights reserved.	en_US
dc.relation.ispartof	Nature Communications	en_US
dc.relation.isbasedon	10.1038/ncomms5342	en_US
dc.subject.mesh	Lung	en_US
dc.subject.mesh	Brain	en_US
dc.subject.mesh	Neurons	en_US
dc.subject.mesh	Muscle Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Drosophila	en_US
dc.subject.mesh	Caenorhabditis elegans	en_US
dc.subject.mesh	Imaging, Three-Dimensional	en_US
dc.subject.mesh	Microsurgery	en_US
dc.subject.mesh	Models, Animal	en_US
dc.subject.mesh	Algorithms	en_US
dc.subject.mesh	Image Processing, Computer-Assisted	en_US
dc.subject.mesh	User-Computer Interface	en_US
dc.title	Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain. © 2014 Macmillan Publishers Limited. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/131815