Full volume super-resolution imaging of thick mitotic spindle using 3D AO STED microscope

Zdankowski, P; McGloin, D; Swedlow, JR

Full volume super-resolution imaging of thick mitotic spindle using 3D AO STED microscope

Zdankowski, P

McGloin, D

Swedlow, JR

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Publication Type:: Journal Article
Citation:: Biomedical Optics Express, 2019, 10 (4), pp. 1999 - 2009
Issue Date:: 2019-04-01

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Field	Value	Language
dc.contributor.author	Zdankowski, P https://orcid.org/0000-0003-3526-3233	en_US
dc.contributor.author	McGloin, D https://orcid.org/0000-0002-0075-4481	en_US
dc.contributor.author	Swedlow, JR https://orcid.org/0000-0002-2198-1958	en_US
dc.date.available	2019-02-11	en_US
dc.date.issued	2019-04-01	en_US
dc.identifier.citation	Biomedical Optics Express, 2019, 10 (4), pp. 1999 - 2009	en_US
dc.identifier.uri	http://hdl.handle.net/10453/132168
dc.description.abstract	© 2019 Optical Society of America under the terms of the OSA Open Access Publishing. Stimulated emission depletion (STED) nanoscopy is one of a suite of modern optical microscopy techniques capable of bypassing the conventional diffraction limit in fluorescent imaging. STED makes use of a spiral phase mask to enable 2D super-resolution imaging whereas to achieve full volumetric 3D super-resolution an additional bottle-beam phase mask must be applied. The resolution achieved in biological samples 10 μm or thicker is limited by aberrations induced mainly by scattering due to refractive index heterogeneity in the sample. These aberrations impact the fidelity of both types of phase mask, and have limited the application of STED to thicker biological systems. Here we apply an automated adaptive optics solution to correct the performance of both STED masks, enhancing robustness and expanding the capabilities of this nanoscopic technique. Corroboration in terms of successful high-quality imaging of the full volume of a 15μm mitotic spindle with resolution of 50nm x 50nm x 150nm achieved in all three dimensions is presented.	en_US
dc.relation.ispartof	Biomedical Optics Express	en_US
dc.relation.isbasedon	10.1364/BOE.10.001999	en_US
dc.rights	© 2019 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.	en_US
dc.title	Full volume super-resolution imaging of thick mitotic spindle using 3D AO STED microscope	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	10	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	0205 Optical Physics	en_US
utslib.for	0912 Materials Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Electrical and Data Engineering
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

© 2019 Optical Society of America under the terms of the OSA Open Access Publishing. Stimulated emission depletion (STED) nanoscopy is one of a suite of modern optical microscopy techniques capable of bypassing the conventional diffraction limit in fluorescent imaging. STED makes use of a spiral phase mask to enable 2D super-resolution imaging whereas to achieve full volumetric 3D super-resolution an additional bottle-beam phase mask must be applied. The resolution achieved in biological samples 10 μm or thicker is limited by aberrations induced mainly by scattering due to refractive index heterogeneity in the sample. These aberrations impact the fidelity of both types of phase mask, and have limited the application of STED to thicker biological systems. Here we apply an automated adaptive optics solution to correct the performance of both STED masks, enhancing robustness and expanding the capabilities of this nanoscopic technique. Corroboration in terms of successful high-quality imaging of the full volume of a 15μm mitotic spindle with resolution of 50nm x 50nm x 150nm achieved in all three dimensions is presented.

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http://hdl.handle.net/10453/132168