Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum

Szczepanek, S; Frasca, JS; Schumacher, V; Liao, X; Padula, M; Djordjevic, S; Geary, S

Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum

Szczepanek, S Frasca, JS Schumacher, V Liao, X Padula, M Djordjevic, S Geary, S

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Publisher:: Amer Soc Microbiology
Publication Type:: Journal Article
Citation:: Szczepanek S et al. 2010, 'Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum', Amer Soc Microbiology, vol. 78, no. 8, pp. 3475-3483.
Issue Date:: 2010

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Field	Value	Language
dc.contributor.author	Szczepanek, S	en_US
dc.contributor.author	Frasca, JS	en_US
dc.contributor.author	Schumacher, V	en_US
dc.contributor.author	Liao, X	en_US
dc.contributor.author	Padula, M	en_US
dc.contributor.author	Djordjevic, S	en_US
dc.contributor.author	Geary, S	en_US
dc.date.accessioned	2011-02-07T06:20:49Z
dc.date.available	2011-02-07T06:20:49Z
dc.date.issued	2010	en_US
dc.identifier	2009006776	en_US
dc.identifier.citation	Szczepanek S et al. 2010, 'Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum', Amer Soc Microbiology, vol. 78, no. 8, pp. 3475-3483.	en_US
dc.identifier.issn	0019-9567	en_US
dc.identifier.other	C1	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13335
dc.description.abstract	Many lipoproteins are expressed on the surfaces of mycoplasmas, and some have been implicated as playing roles in pathogenesis. Family 2 lipoproteins of Mycoplasma pneumolliae have a conserved "mycoplasma lipoprotein X" central domain and a "mycoplasma lipoprotein 10" C-terminal domain and are dill'erentially expressed in response to environmental conditions. Homologues of family 2 lipoproteins are Mycoplasma specific and include the lipoprotein of Mycoplasma gallisepticum, encoded by the MGA0674 gene. Comparative transcriptomic analysis of the M. gallisepticum live attenuated vaccine strain F and the virulent strain R1ow, reported in this study, indicated that MGA0674 is one of several dili'erentially expressed genes. The MGA0674- encoded lipoprotein is a proteolytically processed, immunogenic, TX-1l4 detergent-phase protein which appears to have antigenic divergence between field strains Rtow and S6. We examined the virulence of an R10w aMGA0674 mutant (PIH9) in vivo and observed reduced recovery and attenuated virulence in the tracheas of experimentally infected chickens. The virulence of two additional R10w aMGA0674mutauts, 2162 and 2204, was assessed in a second in vivo virulence experiment. These mutants exhibited partial to complete attenuation ill vivo, but recovery was observed more frequently. Since only Mycoplasma species harbor homologues of MGA0674, the gene product has been renamed "Mycoplasma-specific lipoprotein A" (MsIA). Collectively, these data indicate that MslA is an immunogenic lipoprotein exhibiting reduced expression in an attenuated strain and plays a role in M. gallisepticllm virulence.	en_US
dc.publisher	Amer Soc Microbiology	en_US
dc.relation.ispartof	Verified OK	en_US
dc.relation.ispartof	Infection And Immunity	en_US
dc.relation.isbasedon	http://dx.doi.org/10.1128/IAI.00154-10	en_US
dc.subject.classification	Microbiology	en_US
dc.title	Identification of lipoprotein MsIA as a neoteric virulence factor of Mycoplasma gallisepticum	en_US
dc.type	Journal article
utslib.citation.volume	78	en_US
utslib.citation.number	8	en_US
utslib.location	Washington DC, USA	en_US
utslib.citation.startpage	3475	en_US
utslib.citation.endpage	3483	en_US
utslib.identifier.org	SCI.Institute for Biotechnology of Infectious Diseases	en_US
utslib.for	060500	en_US
utslib.percentage	000050	en_US
utslib.copyright.status	closed_access

Abstract:

Many lipoproteins are expressed on the surfaces of mycoplasmas, and some have been implicated as playing roles in pathogenesis. Family 2 lipoproteins of Mycoplasma pneumolliae have a conserved "mycoplasma lipoprotein X" central domain and a "mycoplasma lipoprotein 10" C-terminal domain and are dill'erentially expressed in response to environmental conditions. Homologues of family 2 lipoproteins are Mycoplasma specific and include the lipoprotein of Mycoplasma gallisepticum, encoded by the MGA0674 gene. Comparative transcriptomic analysis of the M. gallisepticum live attenuated vaccine strain F and the virulent strain R1ow, reported in this study, indicated that MGA0674 is one of several dili'erentially expressed genes. The MGA0674- encoded lipoprotein is a proteolytically processed, immunogenic, TX-1l4 detergent-phase protein which appears to have antigenic divergence between field strains Rtow and S6. We examined the virulence of an R10w aMGA0674 mutant (PIH9) in vivo and observed reduced recovery and attenuated virulence in the tracheas of experimentally infected chickens. The virulence of two additional R10w aMGA0674mutauts, 2162 and 2204, was assessed in a second in vivo virulence experiment. These mutants exhibited partial to complete attenuation ill vivo, but recovery was observed more frequently. Since only Mycoplasma species harbor homologues of MGA0674, the gene product has been renamed "Mycoplasma-specific lipoprotein A" (MsIA). Collectively, these data indicate that MslA is an immunogenic lipoprotein exhibiting reduced expression in an attenuated strain and plays a role in M. gallisepticllm virulence.

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http://hdl.handle.net/10453/13335